Immunohistochemistry analysis of human kidney tissue using CSB-PA614264LA01HU at dilution of 1:100
Immunofluorescent analysis of Hela cells using CSB-PA614264LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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用途:
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Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane.
基因功能參考文獻:
the increased expression of DLG1 may be predictive of an unfavorable prognosis in colorectal cancer. PMID: 29263038
By fluorescence resonance energy transfer (FRET), we detected the direct binding of Tax to DLG1 within the cell. The interaction specifically affects the cellular distribution of not only DLG1, but also Tax. The aggregates distribute into the Golgi apparatus in spatial association with the microtubule-organizing center (MTOC). PMID: 29168728
These data support diverging presynaptic and postsynaptic roles of SAP97 N-terminal isoforms in synapse maturation and plasticity PMID: 28244171
Single nucleotide polymorphism in DLG1 gene is associated with schizophrenia. PMID: 28990294
Low expression of DLG1 is associated with Crohn's disease. PMID: 27760079
BAIAP2 is a candidate gene for mediating dendritic spine density abnormalities in schizophrenia. Data suggest that altered DNA methylation in schizophrenia may be a mechanism for schizophrenia-related dendritic spine density reductions. PMID: 28195572
Findings suggest that synapse-associated protein of 97-kDa molecular weight and disrupted in schizophrenia 1 contribute to maintaining Wnt/beta-catenin signaling activity within a homeostatic range by regulating glycogen synthase kinase 3 beta phosphorylation. PMID: 27026592
that Pten, through the Dlg1-binding ability of its PDZ-binding domain , accumulates phosphorylated Eg5 at duplicated centrosomes to establish symmetrical bipolar spindles that properly segregate chromosomes PMID: 27240320
Results suggest that discs large tumour suppressor (DLG1) expression may have an important role in the progression of early dysplastic cervical lesions, giving prognostic information. PMID: 28040505
Reduced cortical expression of a newly identified splicing variant of the DLG1 gene in patients with early-onset schizophrenia was identified. PMID: 26440542
Human papillomavirus oncoproteins E6 and E7 induce the loss of DLG1 from the cell borders and an increase in the level of DLG1 protein, reflecting the pattern observed in cervical lesions. PMID: 26653181
The study provides new evidence for understanding the transcriptional regulation of DLG1 and the changes in DLG1 expression during different biological processes. PMID: 25720117
Trans-homophilic interaction of CADM1 activates PI3K by forming a complex with MAGuK-family proteins MPP3 and Dlg. PMID: 25268382
the synapse-associated protein-97 (SAP97), an excitatory synapse scaffolding element, governs ADAM10 trafficking from dendritic Golgi outposts to synaptic membranes, mediated by a previously uncharacterized protein kinase C. PMID: 25429624
E4-ORF1 interacts with Dlg1 and PI3K to assemble a ternary complex where E4-ORF1 hijacks the Dlg1 oncogenic function to relocate cytoplasmic PI3K to the membrane for constitutive activation. PMID: 24788832
While other domains of SAP97 were involved in forward trafficking of GluN1-3 out of the endoplasmic reticulum (ER), the SH3 domain was necessary and sufficient to block the ER retention. PMID: 25499266
DLG1, XIST, DDX3Y and RPS4Y1 genes can classify samples into different group clearly, and they are regarded as high confidence distinct gene biomarkers of Parkinson disease. PMID: 25275262
Novel genetic variants in the coding regions of DLG1 gene associated with susceptibility to Crohn's disease. PMID: 24937328
These findings indicate that most, if not all, human adenovirus E4-ORF1 proteins share a conserved molecular mechanism of PI3K activation via Dlg1 binding and confer a common capacity to promote oncogenic transformation. PMID: 25253337
MPP3 and Dlg, membrane-associated guanylate kinase homologs (MAGuK) proteins, connect CADM1 with p85 of PI3K by forming a multi-protein complex at the periphery of cells. PMID: 24503895
SAP97 plays a conserved role in regulating 5-HT2AR endocytosis and ERK1/2 signaling, but plays a novel role in regulating 5-HT2AR G protein coupling. PMID: 24989932
the nature of the residue at position -3 in the PDZ regulated whether the beta1-AR was internalized alone or in complex with SAP97. PMID: 24324269
our findings suggest a molecular mechanism by which CASK binding regulates SAP97 conformation and its subsequent sorting and synaptic targeting of AMPARs and NMDARs during trafficking to synapses. PMID: 23864692
The C-terminal tail of DLG1 is bound to the peptide-binding site of an adjacent symmetry-related DLG1 guanylate kinase molecule. PMID: 23624197
Discs large 1 (Dlg1) scaffolding protein participates with clathrin and adaptator protein complex 1 (AP-1) in forming Weibel-Palade bodies of endothelial cells. PMID: 23532850
During anoikis, hScrib and hDlg1 have distinct and opposing functions in human keratinocytes. PMID: 22792261
Dlg is a physiologically relevant regulator of Cx43 in transformed epithelial cells. PMID: 22657348
Data suggest that hDlg may serve as a platform to bring in proximity APC and PTEN tumor suppressor activities. PMID: 22434720
Mutations in the coding sequence of LAMC1 and DLGH1 may not be associated with Mayer-Rokitansky-Kuster-Hauser Syndrome. PMID: 22377151
Knockdown of Dlg1, which binds Kv1.3 channels to tyrosine kinase Lck, abolishes modulation of Kv1.3 channels. PMID: 22378744
Dlg1 has a distinct oncogenic function in the context of HPV induced malignancy, one of the outcomes of which is increased RhoG activity and increased invasive capacity. PMID: 22383878
Data suggest that the effect of Dlg1 on HIV-1 infectivity is at the stage of virus entry. PMID: 22272285
These findings further support the possible involvement of the SAP97 gene variation in the susceptibility to schizophrenia in males and in the genetic basis for sex differences in the disorder PMID: 22225629
E-cadherin is necessary for localization of DLG1 but not phosphorylated MEK2 to the midbody ring during cytokinesis. PMID: 22185284
These findings not only indicate SAP97 as a point of convergence between amyloid cascade and synaptic failure in AD, but also allow a different interpretation of AD which can be now perceived as synaptic trafficking defect pathology. PMID: 20980075
Dlgh1 operates independently of the negative feedback pathway mediated by the related adapter protein Carma1 and thus presents an array of unique targets to selectively manipulate Treg function PMID: 22307621
Data establish the requirement of scaffolding to DLG1 for PKCalpha to promote cellular migration. PMID: 22027822
Results provide new insights into the binding mode of a defined C-terminal segment of APC by the PDZ domains of DLG1. PMID: 21858148
Low-to-moderate penetrance protein coding mutations or non-coding mutations at DLG1 and/or PAK2, or a nearby gene, may reproduce the behavioral characteristics of the 3q29 microdeletion. PMID: 21850710
These findings indicate that binding of NS1 to Scribble and Dlg1 functions to disrupt the cellular tight junction and that this effect likely contributes to the severe disease associated with highly pathogenic H5N1 influenza A viruses. PMID: 21849460
hDlg acts as a MEK2-specific scaffold protein for the ERK signaling pathway. PMID: 21615688
Studies represent first analyses of 5'-UTR of DLG1 transcripts; data suggest that differential expression of alternatively spliced 5'-UTRs with different translational properties result in changes in DLG1 expression. PMID: 21595829
HPV16 E6 association with PDZ domain-containing proteins, MAGI1, Dlg1 or Scrib, stabilized the levels of E6. PMID: 21489588
SAP97 and dystrophin macromolecular complexes determine two pools of cardiac sodium channels Nav1.5 in cardiomyocytes. PMID: 21164104
the higher off rate constants of HPV16 E6, as compared with those of the HPV18 E6 mimic E6L151V, account for its lower affinity toward the SAP97 PDZ domains, in particular for PDZ2 and PDZ3 PMID: 21113079
In response to hyperosmotic stress, p38 also regulates formation of complexes between hDlg and PSF. PMID: 20605917
The Dlg1-MPP7-Mals3 heterotrimer consists of 2 pairs of heterodimeric L27 domains. These 2 dimers are asymmetric due to the large difference between the N- and C-terminal tandem L27 domain of MPP7. PMID: 20702775
The Dlg1 interacts with dynein via the scaffolding protein GKAP and together, Dlg1, GKAP, and dynein control microtubule dynamics and organization near the cell cortex and promote centrosome positioning. PMID: 21041448
these results reveal a previously unreported pathway for hDlg phosphorylation in mitosis and show that ERK5 pathway mediates hDlg cell cycle dependent phosphorylation. PMID: 20643107
These results uncover a crucial function for ezrin, Dlg1 and microtubules in the organization of the immune synapse and TCR signal down-regulation. PMID: 20551903
Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Expressed in the brain (at protein level). Widely expressed, with isoforms displaying different expression pro