DLAT Antibody

說明書

中文名稱：

DLAT兔多克隆抗體
貨號：

CSB-PA084734
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA084734(DLAT Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA084734(DLAT Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: A431, hepg2 and lncap cell, Primary antibody: CSB-PA084734(DLAT Antibody) at dilution 1/800 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds
其他：

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靶點詳情

產品詳情

Uniprot No.：

P10515
基因名：

DLAT
別名：

70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody; Dihydrolipoamide S Acetyltransferase antibody; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial antibody; DLAT antibody; DLTA antibody; E2 antibody; E2 component of pyruvate dehydrogenase complex antibody; M2 antigen complex 70 kDa subunit antibody; mitochondrial antibody; ODP2_HUMAN antibody; PBC antibody; PDC E2 antibody; PDC-E2 antibody; PDCE2 antibody; Pyruvate dehydrogenase complex component E2 antibody; Pyruvate dehydrogenase complex E2 subunit antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Fusion protein of Human DLAT
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:30-1:150

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

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功能：

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
基因功能參考文獻：
1. DLAT interacts with C1QBP in mitochondria. PMID: 26753982
2. ongoing activation of PDCE2-specific B-cells in primary biliary cirrhosis PMID: 25043065
3. Results show that DLAT and ACAT2 as upstream acetyltransferases of K76 and K294 in 6PGD protein. PMID: 25042803
4. These findings were used to identify potentially antigenic sequences within PDC-E2 (an important hepatic autoantigen) that contain a DR0801 motif. PMID: 23543758
5. Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly PMID: 20361979
6. Data suggest that the catalytic site of pyruvate dehydrogenase complex E2 rather than the previously reported lipoyl binding peptide may contain immunodominant epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis. PMID: 20180236
7. a novel HLA-A*0201-restricted epitope PDC-E2 165 to 174 was defined in patients with primary biliary cirrhosis. PMID: 12395322
8. study of facilitated interaction between the pyruvate dehydrogenase kinase isoform 2 and the dihydrolipoyl acetyltransferase PMID: 12816949
9. model of the pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and binding of the E1 and E3 components PMID: 14638692
10. Having found that there is an excellent and almost unique match between the PDC-E2 autoepitope and a sequence in mycobacterial hsp65s, we tested the corresponding peptides for cross-reactivity using sera from 90 Spanish and British PBC patients. PMID: 15120760
11. This study report two unrelated patients with pyruvate dehydrogenase deficiency caused by defects in the E2 subunit. PMID: 16049940
12. epitope specificity of these PDC-E2 autoantibodies was distinctive suggesting that the mechanisms leading to loss of tolerance in the transplantation patients are distinct from primary biliary cirrhosis PMID: 17068145
13. Species specificity in the interaction between hE1beta and hE2 in pyruvate dehydrogenase complex. PMID: 18206651
14. tissue specificity of the autoimmune injury in primary biliary cirrhosis is a consequence of the unique characteristics of HIBECs during apoptosis and can be explained by exposure to immune system of intact immunoreactive PDC-E2 within apoptotic blebs. PMID: 19185000
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相關疾病：

Pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency)
亞細胞定位：

Mitochondrion matrix.
蛋白家族：

2-oxoacid dehydrogenase family
數據庫鏈接：

HGNC: 2896

OMIM: 245348

KEGG: hsa:1737

STRING: 9606.ENSP00000280346

UniGene: Hs.335551

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