DFFB Antibody

中文名稱：

DFFB兔多克隆抗體
貨號：

CSB-PA006738LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA006738LA01HU diluted at 1:10 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA006738LA01HU diluted at 1:10 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) DFFB Polyclonal antibody
Uniprot No.：

O76075
基因名：

DFFB
別名：

CAD antibody; Caspase activated deoxyribonuclease antibody; Caspase activated DNase antibody; Caspase activated nuclease antibody; Caspase-activated deoxyribonuclease antibody; Caspase-activated DNase antibody; Caspase-activated nuclease antibody; CPAN antibody; Deoxyribonuclease III, caspase activated antibody; DFF 40 antibody; DFF-40 antibody; DFF2 antibody; DFF40 antibody; Dffb antibody; DFFB_HUMAN antibody; Didff antibody; DNA fragmentation factor 40 kDa subunit antibody; DNA fragmentation factor subunit beta antibody; DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase) antibody; DNA fragmentation factor, 40 kD beta subunit antibody; DNA fragmentation factor, 40 kD, beta polypeptide antibody; DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase) antibody; OTTHUMP00000003633 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human DNA fragmentation factor subunit beta protein (108-219AA )
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，DFFB Antibody (CSB-PA006738LA01HU)，的標記方式是Non-conjugated。對于DFFB Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA006738LB01HU	DFFB Antibody, HRP conjugated	ELISA
FITC	CSB-PA006738LC01HU	DFFB Antibody, FITC conjugated
Biotin	CSB-PA006738LD01HU	DFFB Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:10-1:100

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
基因功能參考文獻：
1. we show that executioner caspase activation of the apoptotic nuclease CAD/DFF40 is essential for TRAIL-induced mutations in surviving cells. As exposure to chemotherapy drugs also activates apoptotic caspases and presumably CAD, we hypothesized that these pathways may also contribute to the mutagenesis induced by conventional chemotherapy drugs, perhaps augmenting the mutations that arise from direct DNA damage PMID: 28981092
2. Dff40 expression is upregulated in atherosclerotic plaque. PMID: 28007744
3. the low expression levels of DFF40/CAD and the absence of DNA laddering as common molecular traits in glioblastoma PMID: 26755073
4. Data suggest DFF40 expression in breast cancer cell line is involved in drug sensitivity/resistance to doxorubicin; apoptotic cell death due to doxorubicin (a topoisomerase II inhibitor) is enhanced by DFF40 overexpression in breast cancer cell line. PMID: 26529233
5. Combinatorial use of some sulfonamides such as acetazolamide along with increased expression of DFF40 can potently kill tumor cells via apoptosis. PMID: 25086620
6. DFF40/CAD-independent mechanism driving conformational nuclear changes during caspase-dependent cell death PMID: 24838313
7. the highest order of chromatin compaction observed in the later steps of caspase-dependent apoptosis relies on DFF40/CAD-mediated DNA damage by generating 3'-OH ends in single-strand rather than double-strand DNA nicks/breaks PMID: 23430749
8. These results suggest a cooperative activity between CAD and DNAS1L3 to accomplish internucleosomal DNA fragmentation . PMID: 23229555
9. Human papillomavirus type 16 E6 protein inhibits DNA fragmentation via interaction with DNA fragmentation factor 40 PMID: 22609799
10. During apoptotic rearrangement of interchromatin granule clusters, the nuclear matrix (NuMa rearrangement) and chromatin are closely associated. This process occurs in defined stages and depends on the activity of protein phosphatases, caspases and CAD. PMID: 22023725
11. the cytosolic levels of DFF40/CAD are determinants in achieving a complete apoptotic phenotype, including oligonucleosomal DNA degradation. PMID: 22253444
12. Data show that among the 13 SNPs in the 3 genes, only 3 were found to be polymorphic: R196K and K277R in the DFFB gene, and S12L in the EndoG gene, and all 6 SNPs in the FEN-1 gene were entirely monoallelic. PMID: 22011247
13. These data suggest that DFF 40 mediated apoptosis plays a significant role in mediating sepsis induced cellular dysfunction. PMID: 21820410
14. Mutations and aberrantly spliced transcripts for the CAD gene are frequently associated with hepatocellular carcinoma. PMID: 12610505
15. Hsp70 binds free CAD in TCR-stimulated T cells to stabilize and augment its activity. PMID: 12738667
16. CAD involves unrestricted accessibility of chromosomal DNA at the initial phase of apoptosis, followed by its nuclear immobilization that may prevent the release of the active nuclease into the extracellular environment. PMID: 15569712
17. PARP-1 poly(ADP-ribosyl)ation is a terminal event in the apoptotic response that occurs in response to DNA fragmentation and directly influences DFF40 activity PMID: 15703174
18. Interactions identified here between human placenta histone H1 carboxyl-terminal domain and DFF40/CAD target and activate linker DNA cleavage during the terminal stages of apoptosis. PMID: 15910001
19. Our findings of high frequency of Alu-mediated hCAD deletion in human hepatoma underscore the implication of hCAD in hepatocarcinogenesis PMID: 16007181
20. AIF is responsible for stage I nuclear morphology and HMW DNA degradation is a caspase-activated DNase and AIF-independent process PMID: 16049016
21. levels of CAD were significantly higher in the nuclear fraction of temporal lobe epilepsy samples PMID: 16121124
22. DFFB haploinsufficiency from 1p allelic loss is a contributing factor in oligodendroglioma development PMID: 16156899
23. in apoptotic cells, endogenous and exogenous CAD forms limited oligomers, representing the active nuclease PMID: 16204257
24. the N-terminal region was found to be responsible for the requirement of salt for fibril formation PMID: 16428311
25. CAD is downregulated at the mRNA and protein level during the erythroid differentiation in TF-1 cells. PMID: 16529748
26. poly-glutamic acid and heparin inhibit DFF40/CAD, the latter one being highly effective at nanomolar concentrations. The inhibitory poly-anions bind to the nuclease and impair its ability to bind double-stranded DNA. PMID: 16699957
27. These results suggest that CAD is the endogenous endonuclease that mediates internucleosomal DNA degradation in rotenone-induced apoptosis. PMID: 17239993
28. Data suggest that erythroblast chromatin degradation may involve caspase activated DNase and apoptosis inducing factor, enzymes distinct from those active in apoptotic cells. PMID: 17492772
29. changes induced in DNA conformation upon HMG-box binding makes the substrate more accessible to cleavage by DFF40/CAD nuclease and thus may contribute to preferential linker DNA cleavage during apoptosis PMID: 18239742
30. We have found that neither single-stranded DNA, single-stranded RNA, double-stranded RNA nor RNA-DNA heteroduplexes are cleaved by the DFF40/CAD nuclease. PMID: 18283539
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亞細胞定位：

Cytoplasm. Nucleus.
數據庫鏈接：

HGNC: 2773

OMIM: 601883

KEGG: hsa:1677

STRING: 9606.ENSP00000367454

UniGene: Hs.133089

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