DDX4 Antibody

中文名稱：

DDX4兔多克隆抗體
貨號(hào)：

CSB-PA882074LA01HU
規(guī)格：

￥440
圖片：
Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA882074LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA882074LA01HU at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA882074LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) DDX4 Polyclonal antibody
Uniprot No.：

Q9NQI0
基因名：

DDX4
別名：

DDX 4 antibody; Ddx4 antibody; DDX4_HUMAN antibody; DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody; DEAD box protein 4 antibody; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody; MVH antibody; Probable ATP dependent RNA helicase DDX4 antibody; Probable ATP-dependent RNA helicase DDX4 antibody; VASA antibody; Vasa homolog antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Probable ATP-dependent RNA helicase DDX4 protein (1-223AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，DDX4 Antibody (CSB-PA882074LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于DDX4 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA882074LB01HU	DDX4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA882074LC01HU	DDX4 Antibody, FITC conjugated
Biotin	CSB-PA882074LD01HU	DDX4 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
基因功能參考文獻(xiàn)：
1. Four sterility-related genes, including BOLL, DDX4, HORMAD1, and MAEL, were found to have increased methylation at CpGs of the promoter regions and decreased mRNA expressions in nonobstructive azoospermia and hypospermatogenesis (HS) testis and are believed to be associated with HS. PMID: 28342926
2. positivity for DDX4 gene was detected in 17 of 39 males with nonobstructive azoospermia which was due to maturation arrest in 35.3% of cases, due to incomplete Sertoli cell only in 23.5% and due to complete Sertoli cell only in 41.2%. PMID: 28000927
3. Results suggest that DDX4 positively regulates cell cycle progression of diverse somatic-derived blood cancer cells, implying its broad contributions to the cancer cell phenotype. PMID: 28612512
4. DDX4 cell-free seminal mRNA was absent in 44% of Sertoli cell-only syndrome cases diagnosed by testicular histopathology, but present in all patients presenting with maturation arrest or hypospermatogenesis PMID: 27211570
5. The 20-mer peptide (residues 228-247) of human DDX-4, an ATP-dependent RNA helicase known to regulate germ cell development, binds to a unique shallow binding surface on RanBPM formed by highly conserved loops on the surface of the beta-sheet with two aspartates on one end, a positive patch on the opposite end, and a tryptophan lining at the bottom of the surface. PMID: 27622290
6. Data show that intrinsically disordered N terminus of DEAD-box RNA helicase Ddx4 forms organelles in HeLa cells and in vitro. PMID: 25747659
7. this study reports the first analysis and comparison of the expression patterns and colocalization of DDX4 and CD133 in 59 ovarian cancer patients via tissue microarray and immunofluorescence analysis to identify the expression patterns and relationship of those two markers in ovarian cancer cells. PMID: 24727449
8. Data suggest that VASA shows differential expression in ovary; in resting primordial follicle reserve in childhood/pre-puberty, VASA is associated with Balbiani's body space in germ cell; once follicle enters growing pool, VASA is undetectable. PMID: 23315064
9. Vasa/Ddx4 gene can be involved in regulation of ground and primed states of pluripotency PMID: 22288104
10. overexpression of VASA and or DAZL, in both embryonic and induced pluripotent stem cells promoted differentiation into primordial germ cells, and maturation and progression through meiosis was enhanced. PMID: 22162380
11. Report the generation/characterization of embryonic stem cell lines carrying a VASA-pEGFP-1 reporter construct that expresses GFP in a population of differentiating cells showing expression of characteristic markers of primordial germ cells. PMID: 19937754
12. marker for normal and malignant germ cells PMID: 11850529
13. The expression of VASA mRNA and protein was significantly decreased in the sperm of oligozoospermic men. PMID: 17486274
14. VASA showed a differential pattern of expression throughout the differentiation and proliferative phase and prophase I to finally associate with Balbiani's body in primordial and primary follicles. PMID: 18534994
15. Over-expression of VASA abrogates the G2 checkpoint, induced by DNA damage, by down-regulating the expression of 14-3-3sigma. Results suggest that VASA may play a role in the progression of ovarian cancer or serve as a valuable marker of tumorigenesis. PMID: 18805576
16. Highly methylated regions of the VASA gene are associated with maturation arrest phenotype in the testis. PMID: 19629140
顯示更多

收起更多
亞細(xì)胞定位：

Cytoplasm. Cytoplasm, perinuclear region.
蛋白家族：

DEAD box helicase family, DDX4/VASA subfamily
組織特異性：

Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 18700

OMIM: 605281

KEGG: hsa:54514

STRING: 9606.ENSP00000424838

UniGene: Hs.223581

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
VIM Recombinant Monoclonal Antibody

Applications: ELISA, IHC, IF

Species Reactivity: Human