DBF4 Antibody

中文名稱：

DBF4兔多克隆抗體
貨號(hào)：

CSB-PA887010LA01HU
規(guī)格：

￥440
圖片：
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA887010LA01HU at dilution of 1:100
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) DBF4 Polyclonal antibody
Uniprot No.：

Q9UBU7
基因名：

DBF4
別名：

Activator of S phase kinase antibody; ASK antibody; CHIF antibody; Chiffon homolog A antibody; DBF4 antibody; DBF4 homolog (S. cerevisiae) antibody; DBF4 homolog antibody; DBF4 type zinc finger containing protein 1 antibody; DBF4 zinc finger A antibody; DBF4 zinc finger antibody; DBF4; S. cerevisiae; homolog of antibody; DBF4-type zinc finger-containing protein 1 antibody; DBF4A antibody; DBF4A_HUMAN antibody; Dumbbell former 4; S. cerevisiae; homolog of antibody; OTTHUMP00000161661 antibody; Protein DBF4 homolog A antibody; ZDBF1 antibody; Zinc finger; DBF-type containing 1 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Protein DBF4 homolog A protein (101-281AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，DBF4 Antibody (CSB-PA887010LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于DBF4 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA887010LB01HU	DBF4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA887010LC01HU	DBF4 Antibody, FITC conjugated
Biotin	CSB-PA887010LD01HU	DBF4 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
基因功能參考文獻(xiàn)：
1. Both CDC7 and DBF4 promoters bind E2F, suggesting that increased E2F activity in RB1 mutant cancers promotes increased DDK expression. Surprisingly, increased DDK expression levels are also correlated with both increased chemoresistance and genome-wide mutation frequencies. PMID: 28448802
2. Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication. PMID: 27341472
3. we propose that phosphorylation of TOP2A by CDC7/DBF4 in early S-phase prevents its localization and/or activity at centromeres, and inhibition of TOP2A function could be relevant to prevent premature separation of centromeric DNA. PMID: 27407105
4. The anti-invasive and cell cycle arrest-inducing effects of nitrogen-containing bisphosphonates are not DBF4 mediated in human breast cancer cells. PMID: 24287290
5. Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint. PMID: 22123827
6. bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. PMID: 21536671
7. Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction PMID: 21036905
8. Data document the role of DBF4 as a key player in nitrogen-containing bisphosphonate-induced cytotoxicity, thus explaining the effects on the cell-cycle. PMID: 19744312
9. The interaction with LEDGF relieves autoinhibition of Cdc7-ASK kinase, imposed by the C terminus of ASK. PMID: 19864417
10. results suggest that E2F regulates the ASK promoter through an atypical mode of recognition of the target site PMID: 12015319
11. The HuDbf4 gene has of 12 exons over a 33-kb region. The MluI cell-cycle box is needed for core promoter activation. The Sp1 upregulator & HES-1 repressor coordinately determine promoter efficiency. Transcription initiations occur at -220, -235 and -245. PMID: 12420215
12. Results demonstrate a DNA damage checkpoint that targets Cdc7/Dbf4 protein kinase. PMID: 12535533
13. Cdc7-Dbf4 kinase efficiently phosphorylates p150. PMID: 16826239
14. Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells. PMID: 16899510
15. Overexpression of Dbf4 abrogates the S checkpoint response to ultraviolet radiation but not ionizing radiation. PMID: 17276990
16. There is the differential regulation of a novel gene, namely ASK/Dbf4, in melanoma and suggest that up-regulation of ASK/Dbf4 is a novel molecular determinant with prognostic relevance that confers a proliferative advantage in cutaneous melanoma. PMID: 17768177
17. Cdc7/Dbf4 kinase activity inhibition affects specific phosphorylation sites on MCM2 in cancer cells PMID: 18286467
18. ASK/Dbf4, a novel cell survival gene in melanoma is transcriptionally regulated by E2F1. PMID: 18503552
19. A strong origin of replication at the DBF4 promoter locus, which contains two initiation zones, two origin recognition complex (ORC) binding sites and two DNase I-hypersensitive regions, is identified. PMID: 18536724
20. increased Cdc7-Dbf4 abundance may be a common occurrence in human malignancies PMID: 18714392
21. These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling. PMID: 19111665
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亞細(xì)胞定位：

Nucleus.
組織特異性：

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 17364

OMIM: 604281

KEGG: hsa:10926

STRING: 9606.ENSP00000265728

UniGene: Hs.485380

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