DAZAP2 Antibody
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中文名稱：DAZAP2兔多克隆抗體
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貨號(hào)：CSB-PA622986LA01HU
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: DAZAP2 antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18, 17, 16, 14, 10, 23 kDa
Observed band size: 18 kDa -
IHC image of CSB-PA622986LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of MCF-7 cells with CSB-PA622986LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) DAZAP2 Polyclonal antibody
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Uniprot No.：
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基因名：DAZAP2
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別名：DAZAP2 antibody; KIAA0058DAZ-associated protein 2 antibody; Deleted in azoospermia-associated protein 2 antibody
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宿主：Rabbit
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反應(yīng)種屬：Human, Rat
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免疫原：Recombinant Human DAZ-associated protein 2 protein (1-168AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
本頁(yè)面中的產(chǎn)品，DAZAP2 Antibody (CSB-PA622986LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于DAZAP2 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:200-1:500 IF 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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基因功能參考文獻(xiàn)：
- methylation of DAZAP2 promoter was involved in downregulation of DAZAP2 in multiple myeloma cells. PMID: 22792345
- In summary we established a new mechanism of IL17RB regulation-Smurf2 dependent degradation of its adaptor protein DAZAP2. PMID: 22070932
- Lys residues might play important roles in regulating the intracellular dynamics of the PRTB protein.K153 in human PRTB is essential for its ubiquitin-dependent degradation PMID: 21274613
- DAZAP2 is downregulated in multiple myeloma samples and it may be a signal molecule in multiple myeloma cells. DAZAP2 is involved in the pathogenesis of MM PMID: 17935665
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亞細(xì)胞定位：Cytoplasm. Nucleus. Note=Predominantly nuclear in macrophages, stimulation of IL17RB with its ligand IL17E induces accumulation in the cytoplasm.
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組織特異性：Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Down-regulated in multiple myeloma.
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數(shù)據(jù)庫(kù)鏈接：
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