CLK1 Antibody

說明書

中文名稱：

CLK1兔多克隆抗體
貨號：

CSB-PA556042
規格：

￥2024
圖片：
Western blot analysis of extracts from 293 cells, using CLK1 antibody.

Immunofluorescence analysis of HUVEC cells, using CLK1 antibody.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CLK1 Polyclonal antibody
Uniprot No.：

P49759
基因名：

CLK1
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from internal of Human CLK1.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:3000
IF	1:100-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.
基因功能參考文獻：
1. We now show that the ability of SRPK1 to mobilize SRSF1 from speckles to the nucleoplasm is dependent on active CLK1. Diffusion from speckles is promoted by the formation of an SRPK1-CLK1 complex that facilitates dissociation of SRSF1 from CLK1 and enhances the phosphorylation of several serine-proline dipeptides in this SR protein PMID: 29335301
2. Data suggest that CLK1 activity in both transformed and normal cells is carefully regulated via CLK1 alternative splicing through both exon 4 skipping and intron 4 retention. CLK1 autoregulates by favoring the expression of truncated isoforms, CLK1T1 and CLKT2 in environmental stress. PMID: 29802995
3. These results thus reveal a large program of CLK1-regulated periodic alternative splicing intimately associated with cell cycle control. PMID: 27015110
4. Global CLK-dependent exon recognition and conjoined gene formation revealed with a novel small molecule inhibitor has been described. PMID: 28232751
5. that mitochondrial Clk1 regulated chemoresistance in glioma cells through AMPK/mTOR/HIF-1alpha mediated glycolysis pathway PMID: 28581641
6. SRPK1 interacts with an RS-like domain in the N terminus of CLK1 to facilitate the release of phosphorylated SR proteins, which then promotes efficient splice-site recognition and subsequent spliceosome assembly. PMID: 27397683
7. Clk1, Clk2 and Clk4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. PMID: 27126587
8. removal of the N-terminus or dilution of CLK1 induces monomer formation and reverses this specificity. CLK1 self-association also occurs in the nucleus PMID: 26443864
9. Nuclear CLK-1 mediates a retrograde signalling pathway that is conserved from Caenorhabditis elegans to humans and is responsive to mitochondrial reactive oxygen species, thus acting as a barometer of oxidative metabolism. PMID: 25961505
10. Data suggest that proline phosphorylation by CLK1/CDC-like kinase 1 (but not by SRPK1/serine/arginine-rich splicing factor kinase 1) regulates conformation and alternative splicing function of SFRS1 (serine/arginine-rich splicing factor 1). PMID: 25529026
11. findings suggest that CLK1-dependent hyperphosphorylation is the result of a general mechanism in which the N-terminus acts as a bridge connecting the kinase domain and the RS domain of the SR protein. PMID: 24869919
12. The data establish a new view of SRSF1 protein regulation in which SRPK1 and CLK1 partition activities based on Ser-Pro versus Arg-Ser placement rather than on N- and C-terminal preferences along the RS domain. PMID: 23707382
13. CLK1 Increases While CLK2 Decreases HIV-1 Gene Expression. PMID: 21682887
14. Clk/Sty is found in the nucleus of several different cell types and is a factor directly involved in splicing control PMID: 12773558
15. ASF/SF2 is phosphorylated by SRPK1 and Clk/Sty PMID: 16223727
16. Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells. PMID: 19168442
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亞細胞定位：

Nucleus.
蛋白家族：

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily
組織特異性：

Endothelial cells.
數據庫鏈接：

HGNC: 2068

OMIM: 601951

KEGG: hsa:1195

STRING: 9606.ENSP00000394734

UniGene: Hs.433732

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