CLEC4E Antibody

中文名稱：

CLEC4E兔多克隆抗體
貨號：

CSB-PA891553LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA891553LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA891553LA01HU diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of U251 cells with CSB-PA891553LA01HU at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CLEC4E Polyclonal antibody
Uniprot No.：

Q9ULY5
基因名：

CLEC4E
別名：

C type lectin domain family 4 member E antibody; C type lectin superfamily member 9 antibody; C-type (calcium dependent carbohydrate recognition domain) lectin superfamily member 9 antibody; C-type lectin domain family 4 member E antibody; C-type lectin superfamily member 9 antibody; CLC4E_HUMAN antibody; CLEC 4E antibody; Clec4e antibody; CLECSF9 antibody; Macrophage inducible C type lectin antibody; Macrophage-inducible C-type lectin antibody; MINCLE antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human C-type lectin domain family 4 member E protein (41-219AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CLEC4E Antibody (CSB-PA891553LA01HU)，的標記方式是Non-conjugated。對于CLEC4E Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA891553LB01HU	CLEC4E Antibody, HRP conjugated	ELISA
FITC	CSB-PA891553LC01HU	CLEC4E Antibody, FITC conjugated
Biotin	CSB-PA891553LD01HU	CLEC4E Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions. Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation. Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue.
基因功能參考文獻：
1. Mincle has the ability to survey mycolate-derived glycolipids from actinomycetes, distinguishing non-pathogenic (e.g. Rhodococcus spp.) and pathogenic (e.g. Mycobacterium tuberculosis) species on the basis of alpha-chain length. PMID: 27714279
2. No association was detected with any of the SNPs analysed and susceptibility to tuberculosis. PMID: 27363694
3. combination adjuvant systems demonstrate markedly different immune activation with age, with combined DC activation via Macrophage-inducible C-type lectin and TLR7/8 representing a novel approach to enhance the efficacy of early-life vaccines. PMID: 27793997
4. We here show that Mincle gene expression was induced in alveolar macrophages and neutrophils in bronchoalveolar lavage fluids of mice and patients with pneumococcal pneumonia PMID: 27923071
5. a nonredundant role for Clec4e in coordinating major biological pathways involved in atherosclerosis PMID: 27587433
6. CLEC4E expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
7. The expression of Mincle increases significantly during the early period of Aspergillus fumigatus infection, while expression of eight corresponding cytokines changes. Mincle, as a pattern recognition receptor, may play a role in the early innate immune response of the corneal resistance against fungus. PMID: 28141595
8. Induction of Mincle by Helicobacter pylori and consequent anti-inflammatory signaling denote a bacterial survival strategy. PMID: 26456705
9. our findings identify mincle as a contributor to the inflammatory response after traumatic brain injury PMID: 25111533
10. results suggest that cholesterol crystals are an endogenous ligand for hMincle and that they activate innate immune responses PMID: 26296894
11. Data indicate that MINCLE receptor is able to mediate the response to trehalose-6,6-dimycolate (TDM) dependent on SYK kinase and CARD9 protein. PMID: 26202982
12. mincle is a fungal receptor that can suppress antifungal immunity and, as such, is a potential therapeutic target. PMID: 24721577
13. These results demonstrated that GroMM is a unique ligand for human Mincle that is not recognized by mouse Mincle. PMID: 24733387
14. Results show the molecular mechanism of glycolipid recognition through C-type lectin receptors, which may provide clues to rational design for effective adjuvants. PMID: 24101491
15. Macrophage-inducible C-type lectin is associated with anti-cyclic citrullinated peptide antibodies-positive rheumatoid arthritis in men. PMID: 22932191
16. Mincle is a potentially critical player in human B cell responses. PMID: 22698596
17. Data indicate an unusual pattern of reciprocal expression of Mincle on peripheral blood monocytes or neutrophils. PMID: 22641025
18. silencing of renal DNaseI gene expression initiates a cascade of inflammatory signals including activation of Toll like receptors and Clec4e, leading to progression of both murine and human lupus nephritis PMID: 22479529
19. Our data suggest that Mincle is induced in adipose tissue macrophages in obesity at least partly through the saturated fatty acid/TLR4/NF-kappaB pathway, thereby suggesting its pathophysiologic role in obesity-induced adipose tissue inflammation. PMID: 21282371
20. progenitor-derived mast cells expressed the macrophage-inducible C-type lectin receptor Mincle, and exposure of these cells to M. sympodialis induced up-regulation of the mRNA expression of Mincle PMID: 20608913
21. Several functional states mediating the interaction of Mincle and yeast at the surface of the macrophage. PMID: 18509109
22. important for the initiation of an innate immune response to Candida albicans PMID: 19120481
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亞細胞定位：

Cell membrane; Single-pass type II membrane protein. Cell projection, phagocytic cup.
組織特異性：

Expressed in monocytes and macrophages.
數據庫鏈接：

HGNC: 14555

OMIM: 609962

KEGG: hsa:26253

STRING: 9606.ENSP00000299663

UniGene: Hs.236516

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