CIDEA Antibody

文獻引用 (1) 說明書

中文名稱：

CIDEA兔多克隆抗體
貨號：

CSB-PA005431LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Mouse liver tissue
All lanes: CIDEA antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa

Immunofluorescent analysis of Hela cells using CSB-PA005431LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescence staining of Hela cell with CSB-PA005431LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with CSB-PA005431LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CIDEA Polyclonal antibody
Uniprot No.：

O60543
基因名：

CIDEA
別名：

CIDEACell death activator CIDE-A antibody; Cell death-inducing DFFA-like effector A antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Synthesized peptide derived from human protein
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CIDEA Antibody (CSB-PA005431LA01HU)，的標記方式是Non-conjugated。對于CIDEA Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA005431LB01HU	CIDEA Antibody, HRP conjugated	ELISA
FITC	CSB-PA005431LC01HU	CIDEA Antibody, FITC conjugated
Biotin	CSB-PA005431LD01HU	CIDEA Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Affinity-purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB,IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

ETTL16-mediated translation of CIDEA promotes non-alcoholic fatty liver disease progression via m6A-dependent manner J Tang,PeerJ,2022

產品評價

靶點詳情

功能：

Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.
基因功能參考文獻：
1. Cidea, unexpectedly, functions molecularly as an indirect inhibitor of thermogenesis via inhibition of UCP1 activity. PMID: 27923808
2. These data establish a functional role for Cidea and suggest that, in humans, the association between Cidea levels in white fat and metabolic health is not only correlative but also causative. PMID: 26118629
3. Since Cide-A protein plays a role in the development of metabolic diseases such as obesity, metabolic syndrome, type 2 diabetes and their vascular complications, CIDE -A gene and protein are potential therapeutic targets for these diseases. PMID: 24413203
4. The lowest mean relative of the gene expression for CIDE-A was observed in the group of obese patients with aortic aneurysm and lipid disorders PMID: 25720106
5. the association of tag-single nucleotide polymorphisms and haplotype structures of the CIDEA gene with obesity in a Han Chinese population, was investigated. PMID: 24057179
6. CIDE proteins expression correlate with tumor and survival characteristics in patients with renal cell carcinoma. PMID: 23475172
7. Insulin regulates CIDEA and CIDEC expression via PI3K, and it regulates expression of each protein via Akt1/2-and JNK2-dependent pathways, respectively, in human adipocytes. PMID: 21636835
8. The proportion of subjects with CIDEA) gene V115F (G/T) polymorphism with phenotypes of metabolic syndrome in a Chinese population was significantly higher based on genotype, in the order: GG PMID: 21106268
9. CIDEA binds to liver X receptors and regulates their activity in vitro. PMID: 21315073
10. These data indicate that the carboxy-terminal domain of Cidea directs lipid droplet targeting, lipid droplet clustering, and triglyceride accumulation, whereas the amino terminal domain is required for Cidea-mediated development of enlarged lipid droplets PMID: 20810722
11. CIDEC is essential for the differentiation of adipose tissue PMID: 20945533
12. These results suggest that CIDEA and CIDEC are novel genes regulated by insulin in human adipocytes and may play key roles in the effects of insulin, such as anti-apoptosis and lipid droplet formation. PMID: 20154362
13. Methylation status of CIDEA, HAAO and RXFP3 had significant association with microsatellite instability in endometrial tumors. PMID: 20211485
14. We propose an important and human-specific role for CIDEA in lipolysis regulation and metabolic complications of obesity. PMID: 15919794
15. results support a role for cell death-inducing DFFA (DNA fragmentation factor-alpha)-like effector A (CIDEA) alleles in human obesity PMID: 16186410
16. deglycosylation of CIDE-A correlated with enhanced nuclear export of the protein, and that high level of nonglycosylated CIDE-A inhibited TGFbeta1-dependent cell death. PMID: 17080483
17. No dysregulation of CIDEA expression was observed in individuals with the metabolic syndrome PMID: 17895319
18. The expression of the CIDE-A gene was regulated by CpG methylation of the promoter region. PMID: 18033804
19. F allele of the CIDEA gene may serve as a risk factor for phenotypes related to metabolic syndrome in Japanese men. PMID: 18328351
20. Cidea and other lipid droplet proteins define a novel, highly regulated pathway of triglyceride deposition in human WAT. PMID: 18509062
21. CIDEa is sequestered in mitochondria while transfer of this potentially dangerous protein from mitochondria into nucleus intensifies or even initiates apoptosis. PMID: 18645223
22. CIDEA is involved in adipose tissue loss in cancer cachexia and this may, at least in part, be due to its ability to inactivate PDC, thereby switching substrate oxidation in human fat cells from glucose to FAs. PMID: 19010897
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相關疾病：

In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin-resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.
亞細胞定位：

Lipid droplet. Nucleus.
組織特異性：

Expressed in omental and subcutaneous adipose tissue (at protein level).
數據庫鏈接：

HGNC: 1976

OMIM: 604440

KEGG: hsa:1149

STRING: 9606.ENSP00000320209

UniGene: Hs.249129

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