CHAF1A Antibody
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中文名稱：CHAF1A兔多克隆抗體
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貨號：CSB-PA943110
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規(guī)格：￥2024
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圖片：
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Western blot analysis of extracts from K562 cells and A549 cells, using CAF1A antibody.
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) CHAF1A Polyclonal antibody
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Uniprot No.：
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基因名：CHAF1A
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宿主：Rabbit
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反應(yīng)種屬：Human,Mouse
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免疫原：Synthesized peptide derived from internal of Human CAF1A.
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免疫原種屬：Homo sapiens (Human)
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克隆類型：Polyclonal
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純化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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濃度：It differs from different batches. Please contact us to confirm it.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA,WB,IHC,IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:3000 IHC 1:50-1:200 IF 1:100-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
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基因功能參考文獻：
- CHAF1A and PCNA are highly expressed in cervical squamous cell carcinoma and associated with the malignancy PMID: 29382432
- Recent work has identified the chromatin assembly factor complex CAF-1 as a potent barrier to cellular reprogramming. In addition, CAF-1 has been implicated in the reversion of pluripotent cells to a totipotent-like state and in various lineage conversion paradigms, suggesting that modulation of CAF-1 levels may endow cells with a developmentally more plastic state. PMID: 28692904
- Chromatin reassembly during double-strand break repair was dependent on the HIRA histone chaperone that is specific to the replication-independent histone variant H3.3 and on CAF-1 that is specific to the replication-dependent canonical histones H3.1/H3.2. PMID: 27269284
- Data show that the high expression of chromatin assembly factor 1, subunit A (p150) (CHAF1A) promotes cell proliferation and inhibits cell apoptosis, suggesting that CHAF1A may be developed as a prognosis biomarker and potential therapeutic target of epithelial ovarian cancer (EOC). PMID: 28286267
- CHAF1A inhibits NEIL1 initiated repair in vitro Subsequent restoration of the chaperone-BER complex in cell, presumably after completion of repair, suggests that histone chaperones sequester the repair complex for oxidized bases in non-replicating chromatin, and allow repair when oxidized bases are induced in the genome. PMID: 27794043
- Loss of CAF1 is associated with increased motility and invasive phenotypes seen in transformed cells. PMID: 27872192
- These data suggest that CAF-1- and ASF1A-H3-H4-dependent deposition of the histone (H3-H4)2 tetramers is compatible with MMR and protects the discontinuous daughter strand from unnecessary degradation by MMR machinery. PMID: 26945061
- Novel functions for a separable domain of the p150 protein, regulating protein and DNA interactions at the nucleolus. PMID: 25057015
- The frequency of positive CHAF1A staining in primary tumor mucosa (45.8% of 203 samples) was significantly elevated compared to normal mucosa (18.7% of samples). Increased expression was associated with cancer stage, tumor invasion and histological grade. PMID: 24845563
- Histone chaperone CHAF1A inhibits differentiation and promotes aggressive neuroblastoma. PMID: 24335960
- CAF1 was hijacked by IE2 to facilitate the replication of the HCMV genome, suggesting chromatin assembly plays an important role in herpesviral DNA synthesis, but also provide a model of the virus-induced chromatin instability through CAF1. PMID: 21445097
- CAF-I-dependent control of degradation of the discontinuous strands during mismatch repair. PMID: 21282622
- these findings suggest an expanded role of p150 as a SUMO2/3-interacting factor, and raise the intriguing possibility that p150 plays a role in promoting delivery of SUMO2/3 or SUMO2/3-modified proteins (or both) on chromatin fibers during replication. PMID: 19919826
- p150 was efficiently phosphorylated by Cdc7-Dbf4 kinase. PMID: 16826239
- ACF generates the characteristic 50- to 60-base-pair internucleosomal spacing in silent chromatin by kinetically discriminating against shorter linker DNAs PMID: 17099699
- rs9352 of CHAF1A was repeatedly includes (78% of the 200 bootstrap samples) in the best set of SNPs. CHAF1A may contribute to gliomagenesis. PMID: 18559551
- p150 directly activates transcription, independently of its histone deposition function. PMID: 19324875
- Data show that human CAF-1 (p150) contains two PCNA interaction peptides, and suggest that these peptides are part of the mechanism that enables CAF-1 to function behind replication forks without interfering with other PCNA-mediated processes. PMID: 19822659
- Altered mRNA expression is associated with prostate cancer recurrence. PMID: 15067324
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亞細胞定位：Nucleus. Note=DNA replication foci.
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蛋白家族：CHAF1A family
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數(shù)據(jù)庫鏈接：
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