CDK5RAP3 Antibody

中文名稱：

CDK5RAP3兔多克隆抗體
貨號：

CSB-PA842691LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: U87 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue
All lanes: CDK5RAP3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57, 47, 32, 60 kDa
Observed band size: 57 kDa

Immunofluorescence staining of HepG2 cells with CSB-PA842691LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CDK5RAP3 Polyclonal antibody
Uniprot No.：

Q96JB5
基因名：

CDK5RAP3
別名：

C53 antibody; CDK5 activator binding protein C53 antibody; CDK5 activator-binding protein C53 antibody; CDK5 regulatory subunit associated protein 3 antibody; CDK5 regulatory subunit-associated protein 3 antibody; Cdk5rap3 antibody; CK5P3_HUMAN antibody; HSF 27 antibody; HSF27 antibody; IC53 antibody; Ischemic heart CDK5 activator binding protein C53 antibody; LXXLL/leucine zipper containing ARFbinding protein antibody; LZAP antibody; MST016 antibody; OK/SW cl.114 antibody; PP1553 antibody; Protein HSF-27 antibody
宿主：

Rabbit
反應種屬：

Human, Rat
免疫原：

Recombinant Human CDK5 regulatory subunit-associated protein 3 protein (42-292AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CDK5RAP3 Antibody (CSB-PA842691LA01HU)，的標記方式是Non-conjugated。對于CDK5RAP3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA842691LB01HU	CDK5RAP3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA842691LC01HU	CDK5RAP3 Antibody, FITC conjugated
Biotin	CSB-PA842691LD01HU	CDK5RAP3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, in response to endoplasmic reticulum stress. Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation. Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation. Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint. Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation. May also play a role in the rupture of the nuclear envelope during apoptosis. May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1. Required for liver development.; (Microbial infection) May be negatively regulated by hepatitis B virus large envelope protein mutant pre-s2 to promote mitotic entry.
基因功能參考文獻：
1. CDK5RAP3 negatively regulates the beta-catenin signaling pathway by repressing GSK-3beta phosphorylation in gastric neoplasms. PMID: 27793695
2. C53/LZAP protein bound indirectly to the microtubule (MT), and expression of the C53/LZAP cleavage product caused abnormal MT bundling and NE rupture. PMID: 23478299
3. our findings provide the new evidence that overexpression of CDK5RAP3 promotes HCC metastasis via downregulation of p14(ARF). PMID: 22860085
4. LZAP may play an important role in hepatocellular carcinoma progression PMID: 22028922
5. our results strongly suggest that the binding of hepatitis viral pre-S2 LHBs with C53 is a novel negative regulator of the checkpoint response PMID: 21971960
6. Data indicate that IC53 is a positive mediator for colon cancer progression, and IC53-rs2737 may serve as protection from the onset of colorectal cancer. PMID: 21394385
7. Nuclear gamma-tubulin interacts with tumor suppressor protein C53 that is involved in regulation of DNA damage PMID: 21465471
8. the ability of LZAP to alter p38 phosphorylation depended, at least partially, on the p38 phosphatase, Wip1 PMID: 21283629
9. Findings reveal that CDK5RAP3 is widely overexpressed in hepatocellular carcinoma and that overexpression of CDK5RAP3 promotes HCC metastasis through PAK4 activation. PMID: 21385901
10. IC53 is a novel gene, mainly expressed in vascular endothelial cells and mediates cell proliferation PMID: 12054757
11. results strongly indicate that in response to genotoxic stress, Cdk5 activator-binding protein C53(C53) serves as an important regulatory component of DNA damage checkpoint through modulating cyclin dependent kinase 1-cyclin B1 function PMID: 15790566
12. It is suggested that LZAP can regulate ARF biochemical and biological activity; additionally, LZAP has p53-dependent cell-cycle effects that are independent of ARF. PMID: 16173922
13. it is concluded that CDK5RAP3, CCNB2, and RAGE genes may be used as a very reliable biomarkers of lung adenocarcinoma PMID: 17549666
14. LZAP has a role in NF-kappaB regulation and tumor suppression. PMID: 17785205
15. CDK5 regulatory subunit associated protein 3 promotes checkpoint kinase 1 activation and mitotic entry in both unperturbed cell-cycle progression and DNA damage response. PMID: 19223857
16. Endothelium-specific overexpression of human IC53 downregulates endothelial nitric oxide synthase activity and elevates systolic blood pressure in mice. PMID: 19541669
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亞細胞定位：

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族：

CDK5RAP3 family
組織特異性：

Ubiquitously expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
數據庫鏈接：

HGNC: 18673

OMIM: 608202

KEGG: hsa:80279

STRING: 9606.ENSP00000344683

UniGene: Hs.20157

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