CDK12 Antibody

中文名稱：

CDK12兔多克隆抗體
貨號：

CSB-PA882147LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
All lanes: CDK12 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 165, 164, 142 kDa
Observed band size: 165 kDa
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

The CDK12 antibody is a polyclonal IgG antibody developed in a rabbit. The immunogen is the recombinant human CDK12 protein (1309-1490aa). The purity of this CDK12 antibody exceeds 95% using protein G purification. This CDK12 antibody is specifically reactive with human species. The reactivity of this OCLN antibody with the CDK12 protein has been confirmed by ELISA and WB assays.

CDK12 is a kinase enzyme that regulates the expression of genes involved in various cellular processes, including transcription, DNA damage response and repair, and cell cycle regulation. Mutations in the CDK12 gene have been identified in a subset of tumors, including ovarian, breast, and prostate cancers.
產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) CDK12 Polyclonal antibody
Uniprot No.：

Q9NYV4
基因名：

CDK12
別名：

arginine/serine-rich antibody; Cdc2 related kinase arginine/serine rich antibody; CDC2 related protein kinase 7 antibody; Cdc2-related kinase antibody; CDC2-related protein kinase 7 antibody; Cdk12 antibody; CDK12_HUMAN antibody; Cell division cycle 2 related protein kinase 7 antibody; Cell division cycle 2-related protein kinase 7 antibody; Cell division protein kinase 12 antibody; CRK7 antibody; CRKR antibody; CrkRS antibody; Cyclin-dependent kinase 12 antibody; EC 2.7.11.22 antibody; hCDK12 antibody; kiaa0904 antibody; Pksc antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Cyclin-dependent kinase 12 protein (1309-1490AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產(chǎn)品，CDK12 Antibody (CSB-PA882147LA01HU)，的標記方式是Non-conjugated。對于CDK12 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產(chǎn)品名稱	應用
HRP	CSB-PA882147LB01HU	CDK12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA882147LC01HU	CDK12 Antibody, FITC conjugated
Biotin	CSB-PA882147LD01HU	CDK12 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
基因功能參考文獻：
1. Cyclin K expression positively correlates with cell proliferation; knockdown of cyclin K or its cognate kinase CDK12 prevents the assembly of prereplicative complex in the G1 cell cycle phase. PMID: 29760377
2. CDK12 regulates prereplicative complex assembly to promote mammalian cell proliferation. PMID: 29760377
3. CDK12 gene amplification can contribute to the pathogenesis of breast cancer. PMID: 28334900
4. BRCA1 downregulation combined with CHK1 inhibition induced excessive amounts of DNA damage, resulting in an inability to complete the S-phase. Therefore, we suggest CHK1 inhibition as a strategy for targeting BRCA1- or CDK12-deficient tumors. PMID: 29025359
5. In Ovarian Cancer patients CDK12 loss is consistently associated with a particular genomic instability pattern characterized by hundreds of tandem duplications PMID: 26787835
6. Structures of CDK12/CycK complexes solved in the presence of AMP-PNP. PMID: 26597175
7. Data show that most mutations prevent formation of the cyclin-dependent kinase 12 (Cdk12)/cyclin K (CycK) complex, rendering the kinase inactive. PMID: 25712099
8. CDK12 affects RNA processing events in two distinct ways: Indirectly through generating factor-binding phospho-epitopes on the CTD of elongating RNAPII and directly through binding to specific factors PMID: 25429106
9. CDK12 and CDK13 losses in HCT116 cells preferentially affect expression of DNA damage response. PMID: 25561469
10. CDK12 plays an important role in cotranscriptional processing of c-FOS transcripts PMID: 25384976
11. CDK12 has properties that should confirm interest in its use as a biomarker. PMID: 24240700
12. many CDK12 mutations are an unrecognized cause of HR defects in ovarian cancers PMID: 24554720
13. CDK12 interacts with cyclin K and is required to maintain embryonnic stem cell self-renewal. PMID: 22547058
14. Cyclin K1 is the primary cyclin partner for CDK12/CrkRS and it is required for activation of CDK12/CrkRS to phosphorylate the C-terminal domain of RNA Pol II. PMID: 22988298
15. through regulation of expression of DNA damage response genes, CycK/Cdk12 protects cells from genomic instability PMID: 22012619
16. Data show that siRNA knockdown of hCDK12 in HeLa cells results in alterations in the CTD phosphorylation state. PMID: 20952539
17. CRK7 modifies MAP kinases regulation and resistance to estrogen signaling inhibitors in breast cancers. PMID: 19651820
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相關(guān)疾病：

Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.
亞細胞定位：

Nucleus. Nucleus speckle. Note=Colocalized with nuclear speckles throughout interphase.
蛋白家族：

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
組織特異性：

Ubiquitously expressed.
數(shù)據(jù)庫鏈接：

HGNC: 24224

OMIM: 615514

KEGG: hsa:51755

STRING: 9606.ENSP00000398880

UniGene: Hs.345028

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