CACYBP Antibody

中文名稱：

CACYBP兔多克隆抗體
貨號：

CSB-PA872536LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate
All lanes: CACYBP antibody at 2.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27, 9, 22 kDa
Observed band size: 27 kDa

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA872536LA01HU at dilution of 1:100

Immunofluorescence staining of U251 cells with CSB-PA872536LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating CACYBP in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA872536LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA872536LA01HU (8μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (10μg)
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CACYBP Polyclonal antibody
Uniprot No.：

Q9HB71
基因名：

CACYBP
別名：

CacyBP antibody; Calcyclin binding protein antibody; Calcyclin-binding protein antibody; CYBP_HUMAN antibody; GIG 5 antibody; GIG5 antibody; Growth inhibiting gene 5 antibody; Growth inhibiting gene 5 protein antibody; hCacyBP antibody; MGC87971 antibody; PNAS 107 antibody; PNAS107 antibody; RP1 102G20.6 antibody; S100A6 binding protein antibody; S100A6-binding protein antibody; S100A6BP antibody; Siah-interacting protein antibody; SIP antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Calcyclin-binding protein (2-228AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CACYBP Antibody (CSB-PA872536LA01HU)，的標記方式是Non-conjugated。對于CACYBP Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA872536LB01HU	CACYBP Antibody, HRP conjugated	ELISA
FITC	CSB-PA872536LC01HU	CACYBP Antibody, FITC conjugated
Biotin	CSB-PA872536LD01HU	CACYBP Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
基因功能參考文獻：
1. CacyBP expression is regulated by E2F1, EGR1, and CREB transcription factors in colorectal cancer HCT116 cells. PMID: 29197151
2. Results suggest that CacyBP/SIP plays an important role in inhibiting glioma cell migration and invasion through promoting the degradation of cytoplasmic p27. PMID: 29024247
3. Data show that S100 calcium binding protein A6 (S100A6) is required for the Ca2+-dependent nuclear translocation of calcyclin binding protein (CacyBP/SIP) in colon cancer SW480 cells. PMID: 29534068
4. CacyBP/SIP nuclear localization, dependent on S100 protein, suppresses gastric cancer tumorigenesis through beta-catenin degradation and the dephosphorylation of ERK1/2 during the G2 phase. PMID: 29099417
5. Our data have shown for the first time the regulation of CacyBP/SIP gene expression by NFAT1. Since NFAT transcription factors are involved in processes related to immune response, these results indicate potential involvement of CacyBP/SIP in the immune system. PMID: 28526484
6. These results suggest that CacyBP/SIP may be promoting growth of colon cancer cells by enhancing ubiquitin-mediated degradation of p27kip1. PMID: 28196083
7. The biological characteristics and target proteins of CacyBP/SIP and its exact role in various cancers are discussed. Review. PMID: 26873490
8. CacyBP/SIP nuclear translocation contributes to the proliferation of gastric cancer cells, and CacyBP/SIP exerts this effect, at least in part, by stimulating ubiquitin-mediated degradation of p27Kip1. PMID: 27099442
9. CacyBP/SIP plays an important role in inhibiting apoptosis of glioma cells which might be mediated by ERK1/2 signaling pathway. PMID: 26825673
10. CacyBP/SIP is a useful indicator of dis processes in Chronic Lymphocytic Leukemia (CLL) and plays an important role in sustaining the balance of cell proliferation and apoptosis. PMID: 26603518
11. CacyBP/SIP nuclear translocation promotes the proliferation and cell cycle progression of gastric cancer cells. PMID: 25110433
12. Overexpression of CacyBP is associated with glioma. PMID: 24740456
13. This study presents CacyBP as a promising candidate biomarker for colorectal cancer (CRC) metastasis and also sheds light on the underlying molecular mechanism by which CacyBP promotes CRC metastasis. PMID: 23543800
14. CacyBP enhances multidrug resistance of pancreatic cancer cells by regulation of P-gp and Bcl-2. PMID: 23463283
15. These findings reveal a novel function for SNRK in the regulation of colon cancer cell proliferation and beta-catenin signaling. PMID: 22874833
16. different activity of CacyBP/SIP in neuroblastoma NB2a and colon cancer HCT116 cells might affect the ERK1/2 pathway in the differentiation or proliferation processes PMID: 22480271
17. new insight into the interaction between S100 proteins and CacyBP/SIP PMID: 22295074
18. Data show that CacyBP/SIP might develop into another possible therapeutic target. PMID: 21268134
19. Poor cellular differentiation, lymph node invasion, and clinicopathological staging in breast cancer were associated with CacyBP/SIP expression. PMID: 20585948
20. CacyBP/SIP exhibits a phosphatase activity toward ERK1/2 kinases while its E217K mutant does not. PMID: 21110948
21. CACYBP is associated with acute lung injury in mice PMID: 21297076
22. CacyBP regulation of COX-2 expression may play an important role in human breast carcinogenesis PMID: 20878073
23. investigation of interaction with Siah1 PMID: 12421809
24. the translocation of CacyBP during the retinoic acid-induced differentiation of neuroblastoma SH-SY5Y cells suggested that this protein might play a role in neuronal differentiation PMID: 12895292
25. SIP engages Siah1 by means of two elements, both of which are required for mediating beta-catenin destruction in cells PMID: 16085652
26. Taken together, our work showed that CacyBP/SIP, as a novel down-regulated gene in renal cell carcinoma, suppressed proliferation and tumorigenesis of renal cancer cells. PMID: 17400182
27. CacyBP/SIP may be a potential inhibitor of cell growth and invasion in the gastric cancer cell, at least in part through the effect on beta-catenin protein expression and transcriptional activation of Tcf/LEF PMID: 18171983
28. The first study on the CacyBP/SIP expression pattern in a broad range of human normal and tumor tissues. PMID: 18443365
29. CacyBP/SIP protein might play an important role in the process of pancreatic carcinogenesis and high-level CacyBP/SIP expression might be related to the malignant potential of pancreatic cancer. PMID: 18765951
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亞細胞定位：

Nucleus. Cytoplasm. Note=Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
數據庫鏈接：

HGNC: 30423

OMIM: 606186

KEGG: hsa:27101

STRING: 9606.ENSP00000356652

UniGene: Hs.508524

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