Butyrly-HIST1H2BC (K20) Antibody
產(chǎn)品詳情
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產(chǎn)品名稱：
Rabbit anti-Homo sapiens (Human) HIST1H2BC Polyclonal antibody
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Uniprot No.：
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基因名：
HIST1H2BC
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別名：
H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibody; H2BC6 antibody; H2BFH antibody; HIST1H2BE; antibody; H2BC7 antibody; H2BFG antibody; HIST1H2BF; antibody; H2BC8 antibody; H2BFA antibody; HIST1H2BG; antibody; H2BC10 antibody; H2BFK antibody; HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody; Histone H2B.1 A antibody; Histone H2B.a antibody; H2B/a antibody; Histone H2B.g antibody; H2B/g antibody; Histone H2B.h antibody; H2B/h antibody; Histone H2B.k antibody; H2B/k antibody; Histone H2B.l antibody; H2B/l antibody
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宿主：
Rabbit
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反應(yīng)種屬：
Human
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免疫原：
Peptide sequence around site of Butyrly-Lys (20) derived from Human Histone H2B type 1-C/E/F/G/I
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免疫原種屬：
Homo sapiens (Human)
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標(biāo)記方式：
Non-conjugated
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克隆類型：
Polyclonal
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抗體亞型：
IgG
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純化方式：
Antigen Affinity Purified
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濃度：
It differs from different batches. Please contact us to confirm it.
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保存緩沖液：
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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產(chǎn)品提供形式：
Liquid
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應(yīng)用范圍：
ELISA, WB, ICC, IF
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推薦稀釋比：
| Application |
Recommended Dilution |
| WB |
1:100-1:1000 |
| ICC |
1:20-1:200 |
| IF |
1:10-1:100 |
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Protocols：
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儲(chǔ)存條件：
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
靶點(diǎn)詳情
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功能：
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
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亞細(xì)胞定位：
Nucleus. Chromosome.
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蛋白家族：
Histone H2B family
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數(shù)據(jù)庫(kù)鏈接：
Most popular with customers
Western Blot
Immunocytochemistry analysis of CSB-PA010403OA20butHU diluted at 1:30 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010403OA20butHU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
