BRF1 Antibody
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中文名稱：BRF1兔多克隆抗體
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貨號：CSB-PA004268
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規格：￥1090
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圖片：
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Western Blot analysis of Jurkat cells using TFIIIB90-1 Polyclonal Antibody
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其他：
產品詳情
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Uniprot No.：
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基因名：BRF1
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別名：B related factor 1 antibody; B-related factor 1 antibody; BRF antibody; BRF-1 antibody; brf1 antibody; BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) antibody; General transcription factor IIIB, 90kD subunit antibody; GTF3B antibody; hBRF antibody; hTFIIIB90 antibody; RNA polymerase III antibody; subunit 2 antibody; TAF3B2 antibody; TAF3C antibody; TAFIII90 antibody; TATA box binding protein (TBP) associated factor 3C antibody; TATA box binding protein (TBP) associated factor, RNA polymerase III, GTF3B subunit 2 antibody; TATA box-binding protein-associated factor antibody; TBP associated factor, RNA polymerase III, 90kD antibody; TF3B_HUMAN antibody; TF3B90 antibody; TFIIIB90 antibody; Transcription factor IIIB 90 kDa subunit antibody; Transcription initiation factor IIIB antibody
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宿主：Rabbit
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反應種屬：Human,Mouse
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免疫原：Synthesized peptide derived from the Internal region of Human TFIIIB90-1.
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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抗體亞型：IgG
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純化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：WB, IHC, IF, ELISA
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:5000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
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基因功能參考文獻：
- In an analysis of families with a history of colorectal cancer, we associated germline mutations in BRF1 with predisposition to colorectal cancer. Seven of the identified variants (1 detected in 2 families) affected BRF1 mRNA splicing, protein stability, or expression and/or function. PMID: 28912018
- Site-directed mutagenesis combined with kinase assays and specific phosphosite immunodetection identified Ser-54 (S54) and Ser-334 (S334) as PKA target amino acids in vitro and in vivo. Phosphomimetic mutation of the C-terminal S334 markedly increased TIS11b half-life and, unexpectedly, enhanced TIS11b activity on mRNA decay. PMID: 27708140
- Mutations in BRF1 cause severe short stature, remarkably delayed bone age, dysmorphic features, cerebellar hypoplasia and cognitive dysfunction inherited in an autosomal recessive pattern. PMID: 27748960
- Brf1 expression is increased in human HCC cases, which is correlated with shorter survival times. PMID: 26701855
- BRF1 mutations that reduce protein activity cause neurodevelopmental anomalies, suggesting that BRF1-mediated Pol III transcription is required for normal cerebellar and cognitive development. PMID: 25561519
- hnRNP F is a co-factor in a subset of tristetraprolin/BRF1/BRF2-mediated mRNA decay. PMID: 24978456
- these observations are in favor of a cell- and context-dependent regulation of Tis11b by hypoxia, which then contributes to modulation of angiogenesis. PMID: 21832157
- Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression. PMID: 21106530
- results identify a human Pol III isoform and isoform-specific functions in the regulation of cell growth and transformation PMID: 20154270
- CK2 forms a stable complex with TFIIIB and activates RNA polymerase III transcription in human cells. PMID: 11997511
- human small nuclear RNA gene-specific transcription factor IIIB complex de novo on and off promoter PMID: 12016223
- BRF1 accelerated mRNA decay and antagonized the stabilizing effect of PI3-kinase, while mutation of the zinc fingers abolished both function and ARE-binding activity. This approach identifies BRF1 as an essential regulator of ARE-dependent mRNA decay. PMID: 12198173
- Data report that protein kinase B (PKB/Akt) stabilizes ARE transcripts by phosphorylating butyrate response factor (BRF1) at serine 92. PMID: 15538381
- These results suggest a direct role of an RNA polymerase III transcription factor in the targeting process. PMID: 16982688
- depletion of endogenous TTP and BRF-1 proteins, or overexpression of dominant-negative mutant TTP proteins, impairs the localization of reporter AU-rich element mRNAs PMID: 17369404
- Maf1 occupancy of Pol III genes is inversely correlated with that of the initiation factor TFIIIB (subunit Brf1) and Pol III. PMID: 17499043
- the hypo-phosphorylated Rb appeared to be largely sequestered into a complex with Brf1, which resulted in the blockage of Rb function to repress E2F1 transactivation PMID: 17877750
- Brf1 gene was identified in the genome-wide loss-of-function genetic screen as putative tumor suppressor located at 14q32.33. PMID: 17968325
- MK2-mediated inhibition of BRF1 requires phosphorylation at S54, S92, and S203. PMID: 18326031
- deregulation of Brf1 and Brf2 expression could be a key mechanism responsible for the observed deregulation of RNA pol III transcription in cancer cells PMID: 18700021
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相關疾病：Cerebellofaciodental syndrome (CFDS)
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亞細胞定位：Nucleus.
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蛋白家族：TFIIB family
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