ASF1A Antibody

說明書

中文名稱：

ASF1A兔多克隆抗體
貨號：

CSB-PA000941
規(guī)格：

￥1090
圖片：
Western Blot analysis of Jurkat cells using Asf1a Polyclonal Antibody
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

Uniprot No.：

Q9Y294
基因名：

ASF1A
別名：

Anti silencing function 1A antibody; Anti-silencing function protein 1 homolog A antibody; ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody; ASF1 anti silencing function 1 homolog A antibody; asf1a antibody; ASF1A_HUMAN antibody; CCG1 interacting factor A antibody; CCG1-interacting factor A antibody; CGI 98 antibody; CIA antibody; hAsf1 antibody; hAsf1a antibody; hCIA antibody; Histone chaperone ASF1A antibody; HSPC146 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from the Internal region of Human Asf1a.
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

WB, IF, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:200-1:1000
ELISA	1:20000

Protocols：

Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
基因功能參考文獻：
1. ASF1A is aberrantly over-expressed in GIC tumors and plays key roles in GIC development and progression by stimulating the transcription of beta-catenin target genes. PMID: 28625518
2. ASF1a promotes non-homologous end joining repair by facilitating phosphorylation of MDC1 by ATM at double-strand breaks. PMID: 28943310
3. Data show that the ubiquitin-conjugating enzyme E2 RAD6A/B-MDM2 ubiquitin ligase machinery regulates anti-silencing function 1A protein (ASF1A) degradation. PMID: 26336826
4. quaternary complex of histone H3-H4 heterodimer with chaperone ASF1 and the replicative helicase subunit MCM2 PMID: 26186914
5. Thermodynamic analysis of the quaternary complex together with structural modeling support that ASF1 and MCM2 could form a chaperoning module for histones H3 and H4 protecting them from promiscuous interactions. PMID: 25618846
6. Data indicate Tousled-like kinases (TLK1) phosphorylation has an impact on cell cycle proteins Asf1a and Asf1b function. PMID: 24598821
7. findings show that ASF1A, a histone-remodeling chaperone specifically enriched in the metaphase II oocyte, is necessary for reprogramming of adult dermal fibroblasts into undifferentiated induced pluripotent stem cell PMID: 25035411
8. The ATR checkpoint pathway causes a histone chaperone normally associated with the replication fork, ASF1a, to degrade through a CRL1(betaTRCP)-dependent ubiquitination/proteasome pathway, leading to the localized dechromatinization and gene repression. PMID: 24700029
9. Co-depletion of the histone chaperones ASF1a and ASF1b in human cells induces all hallmarks of alternative lengthening of telomeres in both primary and cancer cells. PMID: 24413054
10. Asf1a plays a role in regulating IE genes by assembling chromatin onto histone-free viral DNA by 3 h postinfection with herpes simplex virus 1 PMID: 22951827
11. The authors propose that Codanin-1 acts as a negative regulator of Asf1 function in chromatin assembly. PMID: 22407294
12. Low ASF1A is associated with familial longevity. PMID: 22247756
13. HIRA plays a unique, ASF1a-independent role, which is required for the localization of HP1 PMID: 21347226
14. Identify marks on histones H3-H4 bound to Asf1 and changes induced upon replication stress. PMID: 20227376
15. model is proposed in which the synergism between hAsf1 and CAF-1 for nucleosome formation during DNA repair is achieved through a transient physical interaction allowing histone delivery from Asf1 to CAF-1 PMID: 11897662
16. NMR structure of the conserved core of hAsf1 A PMID: 15213445
17. Data suggest that Asf1 provides cells with a buffering system for histone excess generated in response to stalled replication and explains how cells maintain an "active" histone pool available during recovery from replication stresses. PMID: 15664198
18. Evidence of binding between a histone and one of its chaperones and genetic data suggesting that this interaction is important in both the DNA damage response and transcriptional silencing. PMID: 15840725
19. The N- and C-terminal regions of ASF1a and ASF1b determine the different affinities of these two proteins for HIRA, by contacting regions outside the HIRA B domain. CAF-1 p60 also uses B domain-like motifs for binding to ASF1a. PMID: 16980972
20. Studies provide evidence for TLK1B-mediated phosphorylation of Asf1 triggering DNA repair. PMID: 17054786
21. The structure of the conserved domain of human ASF1A in complex with the C-terminal helix of histone H3 using nuclear magnetic resonance spectroscopy was solved. PMID: 17292837
22. the crystal structure, at 2.7 A resolution, of CIA-I in complex with histones H3 and H4 PMID: 17293877
23. the expression of human ASF1A and ASF1B are upregulated followed by cell proliferation signal, but that of ASF1B is uniquely regulated by transcription factors E2F during cell cycle progression PMID: 17328667
24. data link Asf1 chaperone function, histone supply, and replicative unwinding of DNA in chromatin; proposed that Asf1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an Asf1-(H3-H4)-MCM2-7 intermediate PMID: 18096807
25. ASF1A and ASF1B play a role in the efficiency of nucleosome assembly in vivo in human cells. PMID: 18378699
26. IE63 of VZV preferentially bound to ASF1a, and the amino-terminal 30 amino acids of ASF1a were critical for its interaction with IE63. PMID: 18971269
27. Hpc2-related domain of UBN1, UBN2, and Hpc2p is an evolutionarily conserved HIRA/Hir-binding domain, which directly interacts with the N-terminal WD repeats of HIRA/Hir. PMID: 19029251
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亞細(xì)胞定位：

Nucleus.
蛋白家族：

ASF1 family
組織特異性：

Ubiquitously expressed.
數(shù)據(jù)庫鏈接：

HGNC: 20995

OMIM: 609189

KEGG: hsa:25842

STRING: 9606.ENSP00000229595

UniGene: Hs.292316

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