ARFGEF2 Antibody

說明書

中文名稱：

ARFGEF2兔多克隆抗體
貨號：

CSB-PA599713
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA599713(ARFGEF2 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA599713(ARFGEF2 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 6%SDS-PAGE, Lysate: 50 μg, Lane: Mouse heart tissue, Primary antibody: CSB-PA599713(ARFGEF2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q9Y6D5
基因名：

ARFGEF2
別名：

ARFGEF2 antibody; ARFGEP2 antibody; BIG2Brefeldin A-inhibited guanine nucleotide-exchange protein 2 antibody; Brefeldin A-inhibited GEP 2 antibody; ADP-ribosylation factor guanine nucleotide-exchange factor 2 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthetic peptide of Human ARFGEF2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:200-1:1000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.
基因功能參考文獻：
1. Both phospholipase D activity and vesicular trafficking were required for effects of BIG1 and BIG2 on beta-catenin activation. Levels of PKA-phosphorylated beta-catenin S675 and beta-catenin association with PKA, BIG1, and BIG2 were also diminished after BIG1/BIG2 depletion. PMID: 27162341
2. Description of a novel ARFGEF2 mutation in five related patients presenting with West syndrome, microcephaly, periventricular heterotopia and thin corpus callosum. PMID: 23812912
3. The clinical phenotype associated with mutations in ARFGEF2 is relatively homogeneous in the families report. PMID: 23755938
4. Arf guanine nucleotide-exchange factors BIG1 and BIG2 regulate nonmuscle myosin IIA activity by anchoring myosin phosphatase complex. PMID: 23918382
5. an early acting GEF (GBF1) activates ARFs that mediate recruitment of late acting GEFs (BIG1/2) to coordinate coating events within the pre-Golgi/Golgi/TGN continuum. PMID: 23386609
6. Brefeldin A-inhibited ADP-ribosylation factor activator BIG2 regulates cell migration via integrin beta1 cycling and actin remodeling. PMID: 22908276
7. Up-regulation of ARFGEF2 is associated with the Huntington's disease. PMID: 21309479
8. BIG1 and BIG2 have roles in endomembrane organization PMID: 20360857
9. involvement in molecular mechanisms of vesicular transport PMID: 11665623
10. identification of protein kinase A-anchoring domains PMID: 12571360
11. BIG2 and Exo70 interact in trans-Golgi network and centrosomes, as well as in exocyst structures or complexes that move along microtubules to the plasma membrane. PMID: 15705715
12. ARFGEF2 mRNA was widely expressed in all cortical layers, especially in the neural precursors of the ventricular and subventricular zones during development, with persistent but diminished expression in adulthood. PMID: 16320251
13. Regulates the constitutive release of tumor necrosis factor receptor type 1 exosome-like vesicles from vascular endothelial cells. PMID: 17276987
14. Phosphorylation of BIG1 and BIG2 via PKA and protein phosphatase 1gamma effects vesicular trafficking via alterations in ARF activation. PMID: 17360629
15. COPII is the only coat required for sorting and export from the endoplasmic reticulum exit sites, whereas GBF1 but not BIGs, is required for COPI recruitment, Golgi subcompartmentalization, and cargo progression to the cell surface. PMID: 18003980
16. These observations indicate that BIG2 and BIG1 play redundant roles in trafficking between the trans-Golgi network and endosomes that involves the AP-1 complex. PMID: 18417613
17. both the constitutive and cAMP-induced release of TNFR1 exosome-like vesicles occur via PKA-dependent pathways that are regulated by the anchoring of RIIbeta to BIG2 via AKAP domains B and C PMID: 18625701
18. Results describe a child with a severe choreadystonic movement disorder, bilateral periventricular nodular heterotopia (BPNH), and secondary microcephaly based on compound heterozygosity for two new ARFGEF2 mutations. PMID: 19384555
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相關疾病：

Periventricular nodular heterotopia 2 (PVNH2)
亞細胞定位：

Cytoplasm. Membrane. Golgi apparatus. Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Endosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, dendrite. Cytoplasmic vesicle. Cell junction, synapse. Cytoplasm, cytoskeleton. Note=Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
組織特異性：

Expressed in placenta, lung, heart, brain, kidney and pancreas.
數據庫鏈接：

HGNC: 15853

OMIM: 605371

KEGG: hsa:10564

STRING: 9606.ENSP00000360985

UniGene: Hs.62578

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