AJAP1 Antibody

中文名稱：

AJAP1兔多克隆抗體
貨號：

CSB-PA891947LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Mouse brain tissue, Mouse skeletal muscle tissue
All lanes: AJAP1 antibody at 2.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA891947LA01HU at dilution of 1:100
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品描述：

CUSABIO's product CSB-PA891947LA01HU is a polyclonal antibody generated in the rabbit with the recombinant human AJAP1 protein (308-394AA) immunized. This anti-AJAP1 antibody is an unconjugated IgG. Its purity is greater than 95% using protein G purified. And it only can react with human and mouse AJAP1 protein, which is frequently reduced or lost in various tumors. The abnormal expression of AJAP1 is related to alterations in cell migration, invasion, increased tumor growth, and tumor vascularization. This AJAP1 antibody has passed multiple quality tests, including ELISA, WB, and IHC.
產品名稱：

Rabbit anti-Homo sapiens (Human) AJAP1 Polyclonal antibody
Uniprot No.：

Q9UKB5
基因名：

AJAP1
別名：

Adherens junction-associated protein 1 antibody; Ajap1 antibody; AJAP1_HUMAN antibody; Membrane protein shrew-1 antibody; MOT8 antibody; SHREW-1 antibody; SHREW1 antibody; Transmembrane protein SHREW1 antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human Adherens junction-associated protein 1 protein (308-394AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，AJAP1 Antibody (CSB-PA891947LA01HU)，的標記方式是Non-conjugated。對于AJAP1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA891947LB01HU	AJAP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA891947LC01HU	AJAP1 Antibody, FITC conjugated
Biotin	CSB-PA891947LD01HU	AJAP1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a role in cell adhesion and cell migration.
基因功能參考文獻：
1. Stable overexpression of AJAP1 promotes adherence, decreases invasion of glioma cells through an extracellular-like matrix, and slows migration in the presence of laminin. These observations are reversed by gene knockdown using multiple siRNAs. Additionally, overexpression of AJAP1 decreases colony formation in glioma cells. PMID: 29115565
2. AJAP1 blocked epithelial-to-mesenchymal transition by interacting with beta-catenin and inhibiting its nuclear translocation, which suppressed zinc finger E-box binding homeobox 1 (ZEB1) transcription. PMID: 28383563
3. EGFR/EGFRvIII remodels the cytoskeleton via epigenetic silencing of AJAP1 in glioma cells. PMID: 28634045
4. Reduced Expression of Adherens Junctions Associated Protein 1 is associated with Recurrence of Hepatocellular Carcinoma After Curative Hepatectomy. PMID: 26122373
5. These findings indicate that downregulated AJAP1 transcription may serve as a novel tumor biomarker to predict recurrence of ESCC after esophagectomy. PMID: 26397940
6. Article summarizes and discusses current knowledge that may identify AJAP1 as a tumor suppressor in gliomas. [Review] PMID: 24807315
7. Results show that AJAP1 is dysregulated at an early stage of gliomagenesis and may suppress glioma cell invasion and proliferation, which suggests that AJAP1 may be a potential diagnostic and prognostic marker for gliomas PMID: 24483339
8. AJAP1 suppressed the expression of MAGEA2 and inhibited the transcriptional activity of MAGEA2 in glioma cells. PMID: 24481586
9. We also identified a significant genetic association between caries of the mid- dentition tooth surfaces and AJAP1 (p value = 2e-8), a gene possibly involved in tooth development. PMID: 23064961
10. This work shows the significant loss of expression of AJAP1 in glioblastoma and provides evidence of its role in the highly migratory characteristic of these tumors. PMID: 22241217
11. Both genetic (gene deletion) and epigenetic alterations (promoter methylation) are likely mechanisms that inactivate the putative tumor suppressor AJAP1 in gliomas, which contributes to poor prognosis. PMID: 21439246
12. Data show that overexpression of shrew-1 leads to preformation of an E-cadherin/EGF receptor/HER2/src-kinase/shrew-1 signaling complex and accelerated E-cadherin internalization. PMID: 19515834
13. While a mutation was not detected in the coding region of the SHREW1 gene in oligodendrogliomas, SHREW1 expression restoration resulted in suppression of cell adhesion and migration, thus, SHREW1 inactivation may lead to oligodendroglial tumorigenesis. PMID: 16410724
14. Adaptor protein complex AP-1B is involved in retaining shrew-1 at the basolateral membrane by postendocytic sorting mechanisms. PMID: 16707570
15. shrew-1 has a function in the regulation of cellular invasion, which may involve its interaction with CD147 PMID: 17267690
16. synergism between a signal peptide and a transmembrane segment to mediate plasma membrane localization is essential for structural and/or functional integrity of shrew-1 PMID: 18485053
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亞細胞定位：

Basolateral cell membrane; Single-pass type III membrane protein. Apical cell membrane; Single-pass type III membrane protein. Cell junction, adherens junction. Note=Mainly basolateral. Localization is mediated by AP1M2.
組織特異性：

Expressed in uterus and pancreas (at protein level).
數據庫鏈接：

HGNC: 30801

OMIM: 610972

KEGG: hsa:55966

STRING: 9606.ENSP00000367432

UniGene: Hs.12473

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