ADH5 Antibody

中文名稱：

ADH5兔多克隆抗體
貨號：

CSB-PA001357LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Rat liver tissue
All lanes: ADH5 antibody at 2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa

Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA001357LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA001357LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA001357LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) ADH5 Polyclonal antibody
Uniprot No.：

P11766
基因名：

ADH5
別名：

ADH 3 antibody; ADH5 antibody; ADHX antibody; ADHX_HUMAN antibody; Alcohol dehydrogenase (class III) chi polypeptide antibody; alcohol dehydrogenase 5 (class III) chi polypeptide antibody; Alcohol dehydrogenase 5 antibody; Alcohol dehydrogenase class 3 antibody; Alcohol dehydrogenase class chi chain antibody; Alcohol dehydrogenase class III antibody; Alcohol dehydrogenase class-3 antibody; Alcohol dehydrogenase class-III antibody; class III alcohol dehydrogenase 5 chi subunit antibody; FALDH antibody; FDH antibody; formaldehyde dehydrogenase antibody; Glutathione dependent formaldehyde dehydrogenase antibody; Glutathione-dependent formaldehyde dehydrogenase antibody; GSH-FDH antibody; GSNOR antibody; hydroxymethyllutathione dehydrogenase antibody; S-(hydroxymethyl)glutathione dehydrogenase antibody
宿主：

Rabbit
反應種屬：

Human, Rat
免疫原：

Recombinant Human Alcohol dehydrogenase class-3 protein (14-130AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，ADH5 Antibody (CSB-PA001357LA01HU)，的標記方式是Non-conjugated。對于ADH5 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA001357LB01HU	ADH5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA001357LC01HU	ADH5 Antibody, FITC conjugated
Biotin	CSB-PA001357LD01HU	ADH5 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol.
基因功能參考文獻：
1. Data (including data from studies using knockout and transgenic mice) suggest that obesity and diabetes are accompanied by decreases in GSNOR activity in hepatocytes engendering nitrosative stress; obesity promotes S-nitrosylation of lysosomal proteins in liver, thereby impairing lysosomal enzyme activities and compromising autophagy. PMID: 29074597
2. GSNOR represents the prototype enzyme to disclose how denitrosylation plays a crucial role in tuning NO-bioactivity and how much it deeply impacts on cell homeostasis and human pathophysiology. (Review) PMID: 28533171
3. It was concluded that in HepG2 cells, ADH5 is a source of formate for de novo purine biosynthesis, especially during folate deficiency when folate-dependent formate production is limited. PMID: 28228507
4. GSNOR expression has different effect on neuronal viability in dependence on the stimulus applied, and plays opposite roles in SH-SY5Y models of Parkinson's disease and amyotrophic lateral sclerosis PMID: 26491229
5. ADH5 counteracts neuronal differentiation of neural stem cells and this effect can be reversed by pharmacological inhibition of ADH5. PMID: 24895131
6. study compared individuals occupationally exposed to formaldehyde and controls to effects of XRCC3 Thr241Met, ADH5 Val309Ile and Asp353Glu polymorphisms; ADH5 polymorphisms did not show significant association with genotoxicity biomarkers PMID: 23355119
7. N6022 binds in the GSNO binding pocket like a competitive inhibitor, although in kinetic assays it behaves with a mixed uncompetitive mode of inhibition (MOI) toward GSNO and a mixed competitive MOI toward formaldehyde adduct S-hydroxymethylglutathione. PMID: 22335564
8. biological significance of SNPs rs11568816, rs17028487 and rs13832 PMID: 21920416
9. Data suggest that GSNOR deficiency, through dysregulated S-nitrosylation, may promote hepatocellular carcinoma, possibly by inactivating a DNA repair system. PMID: 20371487
10. Significant associations were found however, for reactions to alcohol with a SNP in ADH5 (rs6827292, p = .001) and a SNP just upstream of ADH5 (rs6819724, p = .0007) that is in strong LD with rs6827292. PMID: 20158305
11. POZ domain of FBI1 represses transcription of ADH5. PMID: 12004059
12. The structural determination of apo, binary alcohol, and inhibitory ternary FDH complexes provides new insight into the enzyme's metal-assisted catalysis and substrate-binding properties. PMID: 12196016
13. Formation of a FDH-S-(hydroxymethyl)glutathione-NADH ternary complex causes movement of glutathione-dependent formaldehyde dehydrogenase catalytic domain toward the coenzyme-binding domain, and a change in active-site zinc coordination. PMID: 12484756
14. No evidence that alcohol dehydrogenase 3genotype modifies risk related to alcohol and lung cancer. PMID: 14608084
15. data suggest that genetic variation in S-nitrosoglutathione reductase (GSNOR) might play a role in asthma susceptibility PMID: 17543375
16. A statistically significant increase of class III alcohol dehydrogenase isoenzymes was found in the sera of pancreatic cancer patients. PMID: 18095160
17. GSNOR inhibitors to be novel tools for regulating nitric oxide bioactivity and assessing the role of SNOs in vivo. PMID: 19596685
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亞細胞定位：

Cytoplasm.
蛋白家族：

Zinc-containing alcohol dehydrogenase family, Class-III subfamily
數據庫鏈接：

HGNC: 253

OMIM: 103710

KEGG: hsa:128

STRING: 9606.ENSP00000296412

UniGene: Hs.78989

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