ADAM23 Antibody

中文名稱：

ADAM23兔多克隆抗體
貨號：

CSB-PA001285LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: ADAM23 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 92, 93 kDa
Observed band size: 92 kDa

IHC image of CSB-PA001285LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA001285LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of 293 cells with CSB-PA001285LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) ADAM23 Polyclonal antibody
Uniprot No.：

O75077
基因名：

ADAM23
別名：

ADAM23; MDC3; Disintegrin and metalloproteinase domain-containing protein 23; ADAM 23; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3; MDC-3
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Disintegrin and metalloproteinase domain-containing protein 23 protein (498-647AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，ADAM23 Antibody (CSB-PA001285LA01HU)，的標記方式是Non-conjugated。對于ADAM23 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA001285LB01HU	ADAM23 Antibody, HRP conjugated	ELISA
FITC	CSB-PA001285LC01HU	ADAM23 Antibody, FITC conjugated
Biotin	CSB-PA001285LD01HU	ADAM23 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

May play a role in cell-cell and cell-matrix interactions. This is a non-catalytic metalloprotease-like protein.
基因功能參考文獻：
1. The relationships between the decreased methylation levels of the SNAI2 and ADAM23 genes and cancer de-differentiation and haematogenous dissemination, respectively, indicate novel functions of those genes in the invasive processes. PMID: 30189837
2. Our results demonstrate that ADAM23 expression is likely involved in the progression of epithelial ovarian cancer PMID: 29921495
3. ADAM23 regulates neuronal differentiation by triggering specific signaling pathways during human neural progenitor cells differentiation. PMID: 28828010
4. these results support the existence of a second mechanism, alternative to inhibition of protein secretion, by which ADLTE-causing LGI1 mutations exert their loss-of-function effect extracellularly, and suggest that interactions of LGI1 with both ADAM22 and ADAM23 play an important role in the molecular mechanisms leading to utosomal dominant lateral temporal epilepsy PMID: 27760137
5. Our data provide evidence that ADAM23 plays a role in suppression of cancer cell progression through interaction with aVb3 integrin, and suggest that downregulation of ADAM23 in SP cells may contribute toward providing a cancer stem cell phenotype PMID: 26800504
6. High ADAM23 gene methylation is associated with gastric tumor aggressiveness. PMID: 25740824
7. quantification of CXCL12 and ADAM23 methylation could be useful for the prediction of advanced stage of BC PMID: 25620615
8. Intratumoral heterogeneity of ADAM23 promotes tumor growth and metastasis through LGI4 and nitric oxide signals. PMID: 24662834
9. The expression level of ADAM23 is likely to be involved in the progression of non-small-cell lung carcinoma, and its downregulation is probably correlated with promoter methylation. PMID: 21429053
10. a SP1 binding site (-202/-190) that binds SP1 at the proximal promoter of human ADAM23 gene was indentified. PMID: 20851106
11. Together, these results indicate that ADAM23 down-regulation by methylation in brain tumors is a rare event and it may help explain why brain tumor metastases are rarely found elsewhere in the body. PMID: 15862898
12. ADAM23 is frequently silenced in gastric cancers by homozygous deletion or aberrant promoter methylation. PMID: 16103878
13. ADAM23 may be down-regulated by aberrant promoter hypermethylation during the progression of colorectal cancer. PMID: 19089928
14. PrP(C) is a novel molecular partner for ADAM23 in the nervous systems. PMID: 19477226
15. Functional role of ADAM23 during lung caner metastatic progression by negatively modulating alpha(v)beta(3) integrin activation. PMID: 19549921
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.; [Isoform Gamma]: Secreted.
組織特異性：

Highly expressed in the brain and weakly expressed in the heart. In the brain, expressed prominently in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole.
數據庫鏈接：

HGNC: 202

OMIM: 603710

KEGG: hsa:8745

STRING: 9606.ENSP00000264377

UniGene: Hs.370287

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