PPA1 Monoclonal Antibody
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中文名稱：PPA1鼠單克隆抗體
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貨號：CSB-MA614884A0m
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: PPA1 antibody at 1:1000
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: 293T whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: U87 whole cell lysate
Secondary
Goat polyclonal to Mouse IgG at 1/20000 dilution
Predicted band size: 33KDa
Observed band size: 33 KDa
Exposure time: 5min -
Immunofluorescence staining of Hela cells with CSB-MA614884A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). -
Overlay Peak curve showing HepG2 cells stained with CSB-MA614884A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. -
Overlay Peak curve showing 293T cells stained with CSB-MA614884A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號：CSB-MA614884A0m PPA1單克隆抗體是針對無機(jī)焦磷酸酶1（PPA1）研發(fā)的高特異性科研試劑，該靶標(biāo)蛋白通過水解無機(jī)焦磷酸鹽參與核苷酸代謝和能量穩(wěn)態(tài)調(diào)控，在細(xì)胞增殖調(diào)控及腫瘤發(fā)生發(fā)展中具有重要研究價值。本產(chǎn)品采用雜交瘤技術(shù)制備，經(jīng)嚴(yán)格驗證可特異性識別人類PPA1蛋白，適用于多種實驗場景包括酶聯(lián)免疫吸附（ELISA）、蛋白質(zhì)印跡（WB）、免疫熒光（IF）及流式細(xì)胞術(shù)（FC）檢測。該抗體具有批次穩(wěn)定性高、種屬特異性明確的特點，可有效支持腫瘤生物學(xué)、代謝疾病機(jī)制、能量信號通路等領(lǐng)域的基礎(chǔ)研究，特別適用于癌癥相關(guān)蛋白互作網(wǎng)絡(luò)分析、亞細(xì)胞定位研究以及分子診斷標(biāo)志物開發(fā)等科研需求。實驗方案適配常規(guī)細(xì)胞裂解液及組織樣本處理流程，為研究者探索PPA1在細(xì)胞周期調(diào)控、線粒體功能中的分子機(jī)制提供可靠工具，滿足多維度實驗設(shè)計的檢測需求。
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產(chǎn)品名稱：Mouse anti-Homo sapiens (Human) PPA1 Monoclonal antibody
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Uniprot No.：
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基因名：
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別名：cytosolic inorganic pyrophosphatase antibody; diphosphate phosphohydrolase antibody; inorganic diphosphatase antibody; inorganic pyrophosphatase 1 antibody; Inorganic pyrophosphatase antibody; IOPPP antibody; IPYR_HUMAN antibody; PP antibody; PP1 antibody; PPA1 antibody; PPase antibody; Pyp antibody; pyrophosphatase, inorganic, 1 antibody; Pyrophosphate phospho hydrolase antibody; Pyrophosphate phospho-hydrolase antibody; SID6-8061 antibody
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宿主：Mouse
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反應(yīng)種屬：Human
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免疫原：Recombinant Human Inorganic pyrophosphatase protein (1-289AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG1
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純化方式：>95%, Protein G purified
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克隆號：1H4E1
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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基因功能參考文獻(xiàn)：
- The p37 controls cortical NuMA levels via the phosphatase PP1 and its regulatory subunit Repo-Man, but it acts independently of Galphai, the kinase Aurora A, and the phosphatase PP2A. PMID: 29222185
- Proteomic profiling identifies the inorganic pyrophosphatase (PPA1) protein as a potential biomarker of metastasis in laryngeal squamous cell carcinoma. PMID: 26948660
- Results show that PPA1 expression is significantly higher in many tumors, especially those of lung and ovarian origin, which suggests that PPA1 plays an important role in carcinogenesis and in the development of some tumors. PMID: 27666431
- PPA1 serves as a potential prognostic biomarker for patients with Ovarian serous carcinoma. PMID: 28202851
- Thus, the Ska complex, specifically the Ska1 C-terminal domain, recruits PP1 to kinetochores to oppose spindle checkpoint signaling kinases and promote anaphase onset. PMID: 26981768
- PPA1 may serve as a potential biomarker of poor prognosis in patients with gastric cancer PMID: 26722435
- PP-1 and Akt have roles in AR-v7 protein expression and activities when AR is functionally blocked PMID: 26378044
- The Newcastle disease virus-induced translation shutoff at late infection times was attributed to sustaining phosphorylation of eIF2a, which is mediated by continual activation of PKR and degradation of PP1. PMID: 26869028
- The human PP1 interaction with the measles V protein is mediated by a conserved PP1-binding motif in the C-terminal region of the V protein. PMID: 25011105
- Measles virus binding to DC-SIGN leads to inhibition of PP1 phosphatase activity and hence inhibition of RIG-I and Mda5 dephosphorylation. PMID: 25011106
- Results identify PP1 as a novel regulator of Cl(-)-HCO3(-) anion exchangers activity which, in concert with CFTR, coordinates events at both apical and basolateral membranes, crucial for efficient HCO3(-) secretion from Calu-3 cells. PMID: 23215877
- PPP1R42 regulation of PP1 function in centrosome separation. PMID: 23718219
- Pyrophosphatase overexpression is associated with invasion, metastasis and recurrence in gastric cancer PMID: 22797819
- PP1gamma is the major histone H3 phosphatase acting on the mitotically phosphorylated residues H3T3ph, H3S10ph, H3T11ph, and H3S28ph. PMID: 21514157
- IRBIT opposes the effects of WNKs and SPAK by recruiting PP1 to the complex to dephosphorylate CFTR and NBCe1-B, restoring their cell surface expression, in addition to stimulating their activities PMID: 21317537
- Data are consistent with the postsynaptic gephyrin scaffold acting as a platform for protein phosphatase 1 (PP1), which regulates gephyrin cluster size by dephosphorylation of gephyrin- or cytoskeleton-associated proteins. PMID: 20206270
- Neither human hexokinase-1 nor human inorganic pyrophosphatase expression segregated concordantly with human cytoplasmic glutamic-oxaloacetic transaminase expression. PMID: 17494625
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亞細(xì)胞定位：Cytoplasm.
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蛋白家族：PPase family
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組織特異性：Expressed ubiquitously.
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