IFNLR1 Monoclonal Antibody

中文名稱：

IFNLR1鼠單克隆抗體
貨號(hào)：

CSB-MA816871A0m
規(guī)格：

￥1320
圖片：
Immunofluorescence staining of Hela cells with（CSB-MA816871A0m）at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of U251 cells with（CSB-MA816871A0m）at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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產(chǎn)品詳情

產(chǎn)品描述：

CUSABIO貨號(hào)：CSB-MA816871A0m IFNLR1單克隆抗體是針對(duì)人干擾素λ受體1（IFNLR1）研發(fā)的高特異性小鼠源雜交瘤抗體，適用于ELISA和免疫熒光（IF）等科研實(shí)驗(yàn)。IFNLR1作為III型干擾素的核心受體，在肝臟、上皮細(xì)胞等組織中廣泛表達(dá)，介導(dǎo)干擾素λ信號(hào)通路，參與抗病毒免疫應(yīng)答及炎癥調(diào)控，與病毒性肝炎、肝纖維化等病理過(guò)程密切相關(guān)。該抗體采用雜交瘤技術(shù)制備，具有高批次穩(wěn)定性和低交叉反應(yīng)性，能夠精準(zhǔn)識(shí)別天然構(gòu)象的IFNLR1蛋白，適用于蛋白質(zhì)相互作用研究、受體表達(dá)定位分析以及干擾素信號(hào)通路機(jī)制探索。在病毒學(xué)、免疫學(xué)及肝臟疾病研究領(lǐng)域，本產(chǎn)品可為體外模型、細(xì)胞實(shí)驗(yàn)等科研場(chǎng)景提供可靠工具，助力病毒感染機(jī)制解析、免疫調(diào)節(jié)藥物開(kāi)發(fā)等前沿課題。通過(guò)ELISA和免疫熒光技術(shù)，研究者可實(shí)現(xiàn)對(duì)IFNLR1表達(dá)水平的定量檢測(cè)或亞細(xì)胞定位觀察，為相關(guān)疾病的分子機(jī)制研究提供關(guān)鍵實(shí)驗(yàn)支持。
產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) IFNLR1 Monoclonal antibody
Uniprot No.：

Q8IU57
基因名：

IFNLR1
別名：

IFNLR1 antibody; IL28RA antibody; LICR2 antibody; Interferon lambda receptor 1 antibody; IFN-lambda receptor 1 antibody; IFN-lambda-R1 antibody; Cytokine receptor class-II member 12 antibody; Cytokine receptor family 2 member 12 antibody; CRF2-12 antibody; Interleukin-28 receptor subunit alpha antibody; IL-28 receptor subunit alpha antibody; IL-28R-alpha antibody; IL-28RA antibody; Likely interleukin or cytokine receptor 2 antibody; LICR2 antibody
宿主：

Mouse
反應(yīng)種屬：

Human
免疫原：

Recombinant IFNLR1 protein (21-228AA)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2a
純化方式：

>95%, Protein A purified
克隆號(hào)：

5G12F3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IF

推薦稀釋比：

Application	Recommended Dilution
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state. Determines the cell type specificity of the lambda interferon action. Shows a more restricted pattern of expression in the epithelial tissues thereby limiting responses to lambda interferons primarily to epithelial cells of the respiratory, gastrointestinal, and reproductive tracts. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium.
基因功能參考文獻(xiàn)：
1. IFN-lambda4 suppressed HIV infection of macrophages. This IFN-lambda4-mediated HIV inhibition was compromised by the antibodies against IFN-lambda receptor complex, IFN-lambdaR1/IL-10R2. PMID: 30247785
2. Significant associations were observed for 4 variants in IFNAR2, IFNLR1 with hepatitis B virus infection, and IFNLR1-rs4649203 was associated with hepatitis B recovery. Moreover, the authors demonstrated the clear relevance of 5 polymorphisms in IFNA1, IFNA2, IFNL4 with hepatocellular carcinoma. PMID: 29080269
3. In this structure, the receptor peptide forms an 85-A-long extended chain, in which both the previously identified box1 and box2 regions bind simultaneously to the FERM and SH2-like domains of JAK1. PMID: 27725180
4. Results show that IFNLR1 box1 and box2 motifs are required for stable complex formation with JAK1 and the crystal structure of JAK1 reveals IFNLR1 box1 binding site in the FERM domain. Also, structure-based mutagenesis reveals IFNLR1 PxxLxF motif that is essential for binding. Structure of JAK1 with IL10RA box1 indicates conservation among class II receptors. PMID: 27133025
5. Selective expression of IFNlambdaR1 was considered to be a plus point towards the targeted anti-viral activity of IFNlambdas but artificial control on its expression will surely make IFNlambdas a better drug with enhanced activity. PMID: 27166550
6. expression of IL-28RA mRNA may be correlated with the pathogenesis of systemic lupus erythematosus PMID: 25911176
7. Polymorphism within the IL-28R1, but not in IL-28B, is associated with benign prostatic hyperplasia and lower urinary tract symptoms in Chinese. PMID: 26400298
8. Results show that the distribution of IL10RB and IL28RA genotypes among the Hepatitis C virus-infected and control groups did not differ significantly. PMID: 24144988
9. This study is the first to show the activation of a type III interferon response in low-risk human papillomavirus positive cervical cells and suggests that the lack of this response may be related to lesion progression. PMID: 24510368
10. IL28RA polymorphism (rs10903035) seems to be implicated in the glucose homeostasis because AA genotype increases the likelihood of IR, but this association was different depending on hepatic fibrosis and HCV genotype. PMID: 24438680
11. Epigenetic reprogramming of IFNLR1-mediated response potentiates antiviral activity and suppresses tumor growth. PMID: 24409098
12. IFN-lambda receptor 1 expression is induced in chronic hepatitis C and correlates with the IFN-lambda3 genotype and with nonresponsiveness to IFN-alpha therapies. PMID: 24752298
13. The rs12980275 AG/GG genotype and rs10903035 G allele remained independently associated with early pegylated interferon and ribavirin failure of hepatitis C in hepatitis C/HIV coinfected patients. PMID: 23565619
14. Plasmacytoid dendritic cells are major producers of IFN-lambda2 (a type III interferon) in response to viral stimulation and also express functional receptors for this cytokine. PMID: 22891284
15. No consistent association is found between IL28RA and multiple sclerosis in a Basque-Spanish population. PMID: 22386267
16. two SNPs in IL28RA are correlated with susceptibility to HCV infection and spontaneous viral clearance, which implicates a primary role of IL28RA in the outcomes of HCV infection PMID: 21742059
17. IFN-lambdaR1 consists of two distinct domains having fibronectin type III topology. PMID: 20934432
18. Although the IFN-lambda1(Bac)-IFN-lambda1R1 and IFN-lambda1(Ins)-IFN-lambda1R1 complexes differed only in the nature of the expression system used for the ligand, their crystallization conditions and crystal forms were quite different PMID: 20057073
19. study supports that IFN-lambdas do not influence every type of cell and that membrane-associated variant IFN-lambda R1 expression is not sufficient to ensure cellular sensitivity toward these cytokines. PMID: 19798076
20. IL-28 and IL-29 interact with a heterodimeric class II cytokine receptor that consisted of IL-10 receptor beta (IL-10Rbeta) and an orphan class II receptor chain, designated IL-28Ralpha. PMID: 12469119
21. distinct receptor complex that is utilized by all three IFN-lambda proteins for signaling and is composed of two subunits, a receptor designated CRF2-12 (also designated as IFN-lambdaR1) and a second subunit, CRF2-4 (also known as IL-10R2) PMID: 12483210
22. interleukin (IL)-28 receptor tyrosine residues have roles in antiviral and antiproliferative activity of IL-29/interferon-lambda 1 PMID: 15166220
23. Results suggested that the g.32349 G>A polymorphism of IL-28RA might be associated with susceptibility to allergic rhinitis. PMID: 16819289
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亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Type II cytokine receptor family
組織特異性：

Widely expressed.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 18584

OMIM: 607404

KEGG: hsa:163702

STRING: 9606.ENSP00000327824

UniGene: Hs.221375

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