CBX3 Monoclonal Antibody

說明書

中文名稱：

CBX3鼠單克隆抗體
貨號：

CSB-MA432292
規格：

￥1090
圖片：
Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1γ Mouse mAb diluted at 1:200

Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1γ Mouse mAb diluted at 1:200

Western blot analysis of 1) Hela Cell Lysate, 2)3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1γ Mouse mAb diluted at 1:1000.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CUSABIO貨號：CSB-MA432292單克隆抗體是針對染色質調控關鍵因子CBX3開發的科研專用檢測工具，該蛋白作為異染色質蛋白1家族成員，在染色質動態修飾和基因沉默機制中發揮重要作用。本產品經嚴格驗證適用于人、小鼠、大鼠三大物種樣本，可兼容Western Blot、免疫組化（IHC）和ELISA三大檢測平臺，其高特異性和穩定性為表觀遺傳學、腫瘤微環境及干細胞分化研究提供可靠保障。在癌癥發生機制探索中，該抗體可有效檢測CBX3在DNA損傷修復通路中的定位變化；在細胞重編程研究中，能精準示蹤蛋白在核內異染色質區域的動態分布。通過優化抗原表位識別區域，產品在保留天然構象識別能力的同時，顯著降低非特異性結合風險，是研究染色質空間結構調控、基因印記現象及表觀遺傳修飾網絡的理想選擇，適用于基因表達譜分析、蛋白質互作研究和病理機制解析等前沿領域。
Uniprot No.：

Q13185
基因名：

CBX3
別名：

CBX 3 antibody; CBX3 antibody; CBX3_HUMAN antibody; Chromobox homolog 3 (HP1 gamma homolog; Drosophila) antibody; Chromobox homolog 3 antibody; Chromobox protein homolog 3 antibody; GAMMA antibody; HECH antibody; Heterochromatin like protein 1 antibody; Heterochromatin protein 1 homolog gamma antibody; Heterochromatin protein HP1 gamma antibody; HP1 gamma antibody; HP1 gamma homolog antibody; HP1Hs gamma antibody; Modifier 2 protein antibody
宿主：

Mouse
反應種屬：

Human,Mouse,Rat
免疫原：

Recombinant Protein of HP-1γ
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
純化方式：

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
克隆號：

23D7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs).
基因功能參考文獻：
1. c-Myc was capable of upregulating HP1gamma by directly binding to the E-box element in the first intron of HP1gamma gene, and the upregulated HP1gamma, in turn, repressed the expression of miR-451a by enhancing H3K9 methylation at the promoter region of miR-451a. PMID: 28967902
2. CBX3 was overexpressed in pancreatic adenocarcinoma, which was associated with poor prognosis. CDK1 knockdown attenuated the cell cycle transition, proliferation and invasion of CBX3-overexpressing PAAD cells. PMID: 29903985
3. CBX3 serves as a positive regulator of aerobic glycolysis via suppressing of the FBP1 in pancreatic cancer cells. PMID: 29678579
4. HP1gamma is present in the nucleus and the cytoplasm interacting with actin, a protein complex that may exert different functions depending on its subcellular localization. PMID: 29208528
5. CBX3 has a role in promoting tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma PMID: 29462646
6. The deletion of CBX3 directly enforces the expression of CDK6 and p21. PMID: 28193906
7. These data indicate that HP1-gamma is a novel epigenetic repressor of epsilon-globin gene expression. PMID: 28154185
8. these data underscore the importance of IDRs 1, 2, and 3 in defining the structural and dynamic properties of HP1gamma, discoveries that have both mechanistic and potentially biomedical relevance. PMID: 26680990
9. In contrast to HP1alpha, HP1gamma could not recognize H3K9me3 in extended nucleosomes. PMID: 26319017
10. this study identified miR-30a as a tumor-suppressive microRNA that targets HP1gamma in vitro and in vivo to specifically suppress the growth of colorectal cancer in mouse xenograft models. PMID: 26333808
11. BARD1 interacts with H3K9me2 through HP1 and plays a critical role in retention of a BRCA1/BARD1 complex at the site of DNA damage. PMID: 25634209
12. CBX3 and CRABP2 expression was markedly increased in lung cancer tissues PMID: 24751108
13. our data suggested that HP1gamma plays an important role in promoting non-small cell lung cancer PMID: 24981246
14. compartmentalized and total HP1gamma protein expression is increased in PCa and that expression correlates with clinical outcomes better than Gleason score. PMID: 23522301
15. HP1gamma affects the spontaneous differentiation potential of P19 cells, and might play major roles in the decision of cell fates in pluripotent stem cells. PMID: 23313480
16. Identification of CBX3 and ABCA5 as putative biomarkers for tumor stem cells in osteosarcoma. PMID: 22870217
17. Loss of CBX3 results in global RNA processing defect. PMID: 22684280
18. The Cbx3 chromodomain binds with comparable affinities to all of the methylated H3K9, H1K26 and G9aK185 peptides. PMID: 22514736
19. HIV-1 Vpr displaces heterochromatin protein 1-alpha and heterochromatin protein 1-gamma from chromatin, resulting in premature chromatid separation. PMID: 21875947
20. we show distinct cell-type- and cancer-stage-associated patterns of key heterochromatin marks: heterochromatic adaptor proteins HP1alpha and HP1gamma, compared with the gammaH2AX marker of endogenously activated DNA damage response. PMID: 20695923
21. HP1gamma localizes to telomeres in S phase, where it is required to establish/maintain cohesion PMID: 21865325
22. this study reveals that human NIPBL is a novel protein recruited to DSB sites, and the recruitment is controlled by MDC1, RNF168 and HP1gamma. PMID: 21784059
23. The Ca2+ channel beta4c subunit interacts with heterochromatin protein 1 gama via a PXVXL binding motif. PMID: 21220418
24. HP1 gamma binds CD44 pre-mRNA inside the variant region of CD44. PMID: 21358630
25. Under oxidative conditions, HP1gamma dimerized and held TIF1beta in a chromatin component and inhibited its repression ability. PMID: 20675861
26. Pax3 represses transcription through a novel mechanism involving competition between corepressor KAP1 and the heterochromatin-binding protein HP1gamma PMID: 16945326
27. Suv39H1, HP1gamma and histone H3Lys9 trimethylation play a major role in chromatin-mediated repression of integrated HIV-1 gene expression. PMID: 17245432
28. In non-differentiated cells, transcriptional intermediary factor TIF1beta/heterochromatin protein HP1 interaction occurs only within euchromatin and involves selectively HP1beta and HP1gamma, but not HP1alpha. PMID: 17381543
29. Overexpression of HP1-GAMMA in wing imaginal discs caused abnormal wings. PMID: 17575412
30. present an investigation of DNA methylation and histone modification marks across the HNRPA2B1-CBX3 locus in primary peripheral blood mononuclear cells (PBMCs) to characterise the chromatin structure that underlies UCOE activity PMID: 18032920
31. Heterochromatin protein gamma coordinately regulates CCN2/CTGF transcription by interacting with nuclear MMP3. PMID: 18172013
32. the spatial proximities among a constellation of functionally related sites that are found within euchromatic regions of the cell nucleus including: HP1gamma, RNA polymerase II, matrin 3, and SAF-A sites PMID: 18618731
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亞細胞定位：

Nucleus.
數據庫鏈接：

HGNC: 1553

OMIM: 604477

KEGG: hsa:11335

STRING: 9606.ENSP00000336687

UniGene: Hs.381189

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