Sheep Interferon γ,IFN-γ ELISA Kit

Sheep Interferon γ,IFN-γ ELISA Kit

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中文名稱：

綿羊γ干擾素(IFN-γ)酶聯免疫試劑盒
貨號：

CSB-E14018Sh
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

綿羊γ干擾素(IFN-γ)酶聯免疫試劑盒（CSB-E14018Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。IFNG即干擾素-γ ，是一種具有多種生物學活性的細胞因子。在免疫調節方面背景深厚，參與機體的抗病毒、抗腫瘤等免疫反應。其研究機制主要圍繞調節免疫細胞功能，促進Th1細胞分化，增強巨噬細胞活性，激活自然殺傷細胞等。試劑盒檢測范圍為5 pg/mL-200 pg/mL，適用于科研場景下對免疫相關機制的探索；可用于評估綿羊在疫苗接種、寄生蟲感染（如旋毛蟲?。┗蚣毦约膊。ㄈ绮剪斒暇。┑葘嶒災Ｐ椭蠺h1型免疫反應的動態變化；適用于比較不同處理條件下免疫調節機制的研究；為畜牧科學、獸醫學及比較免疫學領域的研究者提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
縮寫：

IFNG
Uniprot No.：

P17773
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

5 pg/mL-200 pg/mL
靈敏度：

2.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-102
1:2	Average %	87
	Range %	84-90
1:4	Average %	85
	Range %	82-89
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of sheep IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	86	83-89

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	2.776	2.798	2.787
100	2.088	2.098	2.093
50	1.164	1.187	1.176
25	0.675	0.633	0.654
10	0.264	0.287	0.276
5	0.125	0.127	0.126

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Immunological response of lame sheep to Clinical Interdigital Dermatitis and Footrot: Procalcitonin, Acute phase proteins, and Pro-inflammatory Cytokines W El-Deeb,Comparative immunology, microbiology and infectious diseases,2022
Effect of progesterone on the vaccination and immune response against Chlamydia abortus in sheep A.Murcia-Belmonte, et al,Veterinary Immunology and Immunopathology,2019
Immune response of sheep naturally infected with Haemonchus spp. on pastures with two different nutritional conditions Gustavo Toscan.et al,SEMIN-CIENC AGRAR,2016
The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis El-Deeb W M,.et al,PeerJ,2016
Relationship between Pro-Inflammatory Cytokines, IL-10 Anti-Inflammatory Cytokine and Serum Proteins in Healthy Lambs and with Diarrhea Fern謾ndez A.et al,Pakistan Veterinary Journal,2016

功能：

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
基因功能參考文獻：
1. gammadelta T cells traveling in the skin-draining afferent lymph are effectors that produce IFN-gamma or IL-17 and express high levels of the skin- and inflammation-seeking molecule E-selectin ligand PMID: 23838472
2. structural and functional characterization and role in haemonchiasis resistance PMID: 21419496
3. results suggest that intron 1 of the interferon gamma gene has an important role in resistance to nematode infection in the Texel breed PMID: 16054889
4. Five unique polymorphic patterns were detected and sequence analysis revealed that these patterns represent five different IFNG alleles. PMID: 16820277
亞細胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
組織特異性：

Released primarily from activated T lymphocytes.
數據庫鏈接：

KEGG: oas:443396

UniGene: Oar.457

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.