Sheep C-reactive protein(CRP) ELISA Kit

產(chǎn)品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊C反應蛋白(CRP)酶聯(lián)免疫試劑盒（CSB-E15081Sh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CRP含量。CRP（C反應蛋白）是一種由肝臟合成的蛋白質(zhì)，當體內(nèi)發(fā)生炎癥時，CRP水平會升高。它作為炎癥標志物，能結合肺炎鏈球菌等病原體，激活補體系統(tǒng)，增強白細胞吞噬作用，參與非特異性免疫反應。試劑盒檢測范圍為0.625 μg/mL-40 μg/mL，適用于綿羊模型中炎癥機制研究、疾病進程評估及免疫調(diào)控相關科研實驗，例如感染性疾病模型構建中CRP動態(tài)監(jiān)測、應激反應評估或藥物干預效果分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

CRP
Uniprot No.：

W5PD71
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.625 μg/mL-40 μg/mL
靈敏度：

0.156 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	92-100
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	87-98

回收率：

The recovery of sheep CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	90	84-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.