Sheep 5-hydroxytryptamine/serotonin(5HT/ST) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊5羥色胺/血清素/血清胺(5HT/ST)酶聯(lián)免疫試劑盒（CSB-E14929Sh）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的5HT/ST含量。5HT即5-羥色胺，是人體內(nèi)重要神經(jīng)遞質(zhì)。5HT/ST靶點相關研究聚焦其在調(diào)節(jié)情緒、認知等方面作用機制。通過藥物作用于該靶點，可調(diào)節(jié)5HT水平與功能，用于治療如抑郁癥、焦慮癥等精神疾病，目前仍在不斷深入探索以挖掘更多臨床價值。試劑盒檢測范圍為0.625 ng/mL-10 ng/mL，適用于綿羊模型在神經(jīng)生物學、藥理學及動物行為學研究中的應用，例如評估應激狀態(tài)下神經(jīng)遞質(zhì)動態(tài)變化、藥物干預對血清素系統(tǒng)的影響或消化道功能相關機制探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

5HT/ST
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma
檢測范圍：

0.625 ng/mL-10 ng/mL
靈敏度：

0.312 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep 5HT/ST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	84
	Range %	80-92
1:40	Average %	97
	Range %	91-105
1:80	Average %	96
	Range %	90-101
1:160	Average %	93
	Range %	86-98

回收率：

The recovery of sheep 5HT/ST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

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