Rat urokinase plasminogen activator,uPA ELISA kit

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中文名稱：

大鼠尿激酶型纖溶酶原激活物(uPA)酶聯免疫試劑盒
貨號：

CSB-E07368r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠尿激酶型纖溶酶原激活物(uPA)酶聯免疫試劑盒（CSB-E07368r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PLAU含量。PLAU是一種重要的靶點。它參與多種生理病理過程，與腫瘤細胞的侵襲、轉移密切相關。在研究機制方面，主要聚焦于其酶活性及信號通路，明確它對腫瘤微環境的調控作用，為開發針對性的抗腫瘤藥物指明方向。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于心血管疾病、纖維化疾病或腫瘤相關動物模型中纖溶系統活性的動態監測，以及細胞遷移、組織修復等基礎研究中的uPA表達水平分析，為科研人員探究uPA在病理生理過程中的調控機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit
縮寫：

PLAU
Uniprot No.：

P29598
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	97
	Range %	94-103
1:8	Average %	101
	Range %	96-105

回收率：

The recovery of rat uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	94-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.334	2.186	2.260	2.077
500	1.652	1.625	1.639	1.456
250	1.047	0.997	1.022	0.839
125	0.636	0.688	0.662	0.479
62.5	0.398	0.402	0.400	0.217
31.2	0.325	0.337	0.331	0.148
15.6	0.246	0.232	0.239	0.056
0	0.189	0.177	0.183

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

In vivo evaluation of urokinase-loaded hollow nanogels forsonothrombolysis on suture embolization-induced acute ischemicstroke rat model Yuming Teng.et al,Bioactive Materials,2017
In vivo evaluation of urokinase-loaded hollow nanogels for sonothrombolysis on suture embolization-induced acute ischemic stroke rat model YumingTeng.et al,Bioactive Materials,2017

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功能：

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
數據庫鏈接：

STRING: 10116.ENSRNOP00000014273

UniGene: Rn.6064

Rat urokinase plasminogen activator,uPA ELISA kit

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