Rat soluble E-selectin,sE-selectin ELISA Kit

Rat soluble E-selectin,sE-selectin ELISA Kit

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文獻引用 (9) 說明書下載

中文名稱：

大鼠可溶性E選擇素(sE-selectin)酶聯(lián)免疫試劑盒
貨號：

CSB-E07996r
規(guī)格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠可溶性E選擇素(sE-selectin)酶聯(lián)免疫試劑盒（CSB-E07996r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的SELE含量。SELE即選擇素E，是內(nèi)皮細胞在炎性因子刺激下表達的黏附分子。其背景圍繞炎癥反應，在多種炎癥相關(guān)疾病中表達異常。研究機制表明，它能介導白細胞與內(nèi)皮細胞的黏附、滾動，參與炎癥細胞募集，在炎癥、腫瘤轉(zhuǎn)移等病理過程意義重大。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于心血管疾病、糖尿病并發(fā)癥等炎癥相關(guān)動物模型的機制研究，或體外細胞實驗中內(nèi)皮細胞激活程度的動態(tài)監(jiān)測。為研究炎癥信號通路、藥物干預效果或組織修復機制提供可靠工具，滿足基礎科研中對靶標蛋白定量分析的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
縮寫：

SELE
Uniprot No.：

P98105
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	88-108
1:10	Average %	94
	Range %	91-98
1:20	Average %	96
	Range %	89-100
1:40	Average %	84
	Range %	80-88

回收率：

The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	93-105
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.746	2.645	2.696	2.542
2500	1.976	1.875	1.926	1.772
1250	1.228	1.204	1.216	1.062
625	0.782	0.761	0.772	0.618
312	0.522	0.511	0.517	0.363
156	0.363	0.352	0.358	0.204
78	0.290	0.285	0.288	0.134
0	0.154	0.153	0.154

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model Chernukha IM.et al,Anim Sci J,2018
Impact of age on cardiovascular function, inflammation, and oxidative stress in experimental asphyxial cardiac arrest Secher N.et al,Acta Anaesthesiol Scand.,2017
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest. Troelsen TT.et al,Acta Anaesthesiol Scand.,2015
Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest T. T. TROELSEN et al,Anesthesia & Pain Management,2015
Effects of interactions between various fats and active/passive phases on postprandial inflammation in rats H. Gül Otlu. et al,Biological Rhythm Research,2016
Preserved cerebral microcirculation after cardiac arrest in a rat model Secher N. et al,Microcirculation,2015
Myo-inositol is a promising treatment for the prevention of ovarian hyperstimulation syndrome (OHSS): an animal study Turan GA. et al,Arch Gynecol Obstet,2015
Long-term high-fat consumption leads to downregulation of Akt phosphorylation of eNOS at Ser1177 and upregulation of Sirtuin-1 expression in rat cavernous tissue Tomada I et al,Age (Dordr),2013
Electrical Vagus Nerve Stimulation Attenuates Systemic Inflammation and Improves Survival in a Rat Heatstroke Model Yamakawa K et al,PLoS One,2013

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功能：

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
基因功能參考文獻：
1. The e-selectin/biliverdin reductase pathway participates in the inhibitory process of hypoxia in pulmonary artery endothelial cell apoptosis which is mediated by the mitochondrial-dependent apoptosis pathway. PMID: 27033411
2. Decrease in uterine pinopodes and L-selectin ligand MECA-79 expression during uterine receptivity period by testosterone may cause failure of blastocyst implantation. PMID: 24966906
3. E-selectin blockade similarly inhibits the migration of activated CCR4(negative) and CCR4(positive) CD4 T cells to dermal inflammation. PMID: 22664869
4. The expression of E-selectin is significantly increased in lung tissue of ovariectomized rats, an effect prevented by estradiol but not by progesterone treatment. PMID: 20735828
亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Selectin/LECAM family
數(shù)據(jù)庫鏈接：

KEGG: rno:25544

STRING: 10116.ENSRNOP00000034797

UniGene: Rn.10359

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.