Rat sex hormone-binding globulin (SHBG) ELISA kit
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中文名稱：大鼠性激素結(jié)合球蛋白(SHBG)酶聯(lián)免疫試劑盒
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貨號：CSB-E12118r
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠性激素結(jié)合球蛋白(SHBG)酶聯(lián)免疫試劑盒（CSB-E12118r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的SHBG含量。SHBG（性激素結(jié)合球蛋白），能特異性結(jié)合性激素，調(diào)節(jié)其生物活性和代謝。在研究機制方面，它與體內(nèi)性激素水平相關(guān)，其表達(dá)受多種激素調(diào)節(jié)。SHBG水平變化在代謝性疾病、生殖系統(tǒng)疾病等研究中受關(guān)注，為相關(guān)疾病診治提供潛在靶點。試劑盒檢測范圍為375 ng/mL-6000 ng/mL，適用于科研領(lǐng)域研究SHBG在性激素代謝調(diào)控中的作用；可用于探究性腺疾病模型、激素代謝異常機制、藥物干預(yù)對性激素分布的影響等研究方向，為生殖生理學(xué)、內(nèi)分泌學(xué)及藥理學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
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縮寫：SHBG
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
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檢測范圍：375 ng/mL-6000 ng/mL
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靈敏度：375 ng/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Signal Transduction
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 88 Range % 85-90 1:4 Average % 100 Range % 97-103 1:8 Average % 83 Range % 80-86 -
回收率：
The recovery of rat SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 87 82-91 EDTA plasma (n=4) 95 89-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average 6000 0.204 0.201 0.203 3000 0.336 0.341 0.339 1500 0.789 0.772 0.781 750 1.800 1.806 1.803 375 2.312 2.353 2.333 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- The Ramazzini Institute 13-week pilot study glyphosate-based herbicides administered at human-equivalent dose to Sprague Dawley rats: effects on development and endocrine system Manservisi F, et al,Environmental health,2019
- Effect of Metformin and Flutamide on Insulin, Lipogenic and Androgen-Estrogen Signaling and Cardiometabolic Risk in a PCOS-prone Rodent Model Maria Kupreeva.et al,American Journal of Physiology-Endocrinology and Metabolism,2018
- Curcumin Attenuates Testicular Injury in Rats with Streptozotocin-Induced Diabetes Wenliang Zha.et al,Biomed Research International,2018
- Testosterone regulates the autophagic clearance of androgen binding protein in rat Sertoli cells Ma Y et al,Sci Rep,2015
- Antioxidants inhibition of high plasma androgenic markers in the pathogenesis of ethylene glycol (EG)-induced nephrolithiasis in Wistar rats Naghii MR et al,Urolithiasis,2013
- Prior caloric restriction increases survival of pre-pubertal obese-and PCOS-prone rats exposed to a challenge of time-limited feeding and physical activity. Diane A et al,J Appl Physiol,2013
產(chǎn)品評價
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靶點詳情
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功能：Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
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基因功能參考文獻(xiàn)：
- SHBG may be of functional importance for rapid effects of olfactory steroids on limbic functions including the control of reproductive behaviours through pheromones PMID: 24681170
- Fluorine had no effect on the expression of androgen binding protein and inhibin B mRNA in testis sertoli cells of Sprague Dawley rats. PMID: 21033444
- Upstream stimulatory factors (USF1 and USF2) induce Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation. PMID: 21734262
- Data show that the use of alternative SHBG first exons 1A and 1B differentially inhibits translation from the ATG situated in exon 2, which codes for methionine 30 of transcripts that begin with the exon 1 sequence. PMID: 21079794
- Results suggest that Sertoli cell-derived androgen-binding protein acts on spermatids by modifying the transition protein-1 mRNA level. PMID: 12573823
- A wave of apoptosis during the pre-pubertal period is required for normal spermatogenesis to develop, and our data indicate that this apoptotic wave may be regulated by ABP and/or androgens. PMID: 12831397
- ABP was found in neurons of the magnocellular hypothalamic nuclei, the preoptic region and the lateral hypothalamus. ABP varicosities occurred throughout the hypothalamus, the median eminence and the posterior pituitary. PMID: 14625054
- ABP expression is regulated in differentiated Sertoli cells by the bHLH and CREB family of transcription factors. PMID: 15112319
- Estrogen receptor beta was strongly immunolabeled in pachytene spermatocytes of rat androgen-binding protein transgenic mice and localized in tubular stages in which TUNEL labeling was maximal. PMID: 15215204
- There is a significantly higher level of the Shbg transcript in hypodactylous rats compared to SHR controls. PMID: 15222128
- Intrahypothalamic expression of ABP may be among the mechanisms necessary for rapid actions of steroids on hypothalamic neuroendocrine systems. PMID: 15927785
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亞細(xì)胞定位：Secreted. Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
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組織特異性：Isoform 2 is only expressed in the liver.
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