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Rat secreted phospholipase A2,sPLA2 ELISA Kit

文獻引用 (2) 說明書下載

產品詳情

產品描述：

大鼠分泌型磷脂酶A2(sPLA2)酶聯免疫試劑盒（CSB-E13206r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的sPLA2含量。sPLA2即分泌型磷脂酶 A2，在生物體內廣泛存在。它參與磷脂代謝，能水解膜磷脂產生花生四烯酸和溶血磷脂等。其異常表達與多種疾病如炎癥、心血管病、腫瘤等相關，因此針對 sPLA2 作用機制的研究，能為疾病治療提供新靶點與思路。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，支持科研人員探究sPLA2在疾病模型中的動態變化，例如心血管疾病機制研究、炎癥相關動物實驗或藥物干預效果評估，尤其適用于分析血清/血漿樣本的循環sPLA2水平或組織勻漿中的局部表達差異本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

sPLA2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat sPLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	93
	Range %	87-97
1:4	Average %	91
	Range %	85-97
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of rat sPLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-93
EDTA plasma (n=4)	92	87-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

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