Rat platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa ELISA Kit

Rat platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa ELISA Kit

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中文名稱：

大鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08776r
規(guī)格：

96T/48T
價(jià)格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)酶聯(lián)免疫試劑盒（CSB-E08776r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GPⅡbⅢa/CD41+CD61含量。GPⅡbⅢa/CD41+CD61作為靶點(diǎn)，在血小板功能中至關(guān)重要。參與血小板聚集過程，研究機(jī)制聚焦于通過阻斷該靶點(diǎn)，抑制血小板的聚集與黏附，進(jìn)而為抗血栓藥物研發(fā)提供方向，以防治相關(guān)心血管疾病。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL，適用于多種生物樣本中GP - ⅡbⅢa的精確檢測(cè)，可廣泛用于大鼠模型中血小板功能調(diào)控機(jī)制研究、血栓相關(guān)疾病動(dòng)物模型的病理分析、藥物干預(yù)效果評(píng)價(jià)等科研領(lǐng)域，為探索血小板相關(guān)分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa |platelet membrane glycoprotein 2b3a,GP-2b3a
縮寫：

GPⅡbⅢa/CD41+CD61
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GP-ⅡbⅢa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	89
	Range %	82-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	90
	Range %	85-98

回收率：

The recovery of rat GP-ⅡbⅢa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	98	92-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.176	2.053	2.115	1.921
200	1.855	1.764	1.810	1.616
100	1.542	1.469	1.506	1.312
50	1.236	1.178	1.207	1.013
25	0.837	0.786	0.812	0.618
12.5	0.421	0.393	0.407	0.213
6.25	0.213	0.196	0.205	0.011
0	0.198	0.190	0.194

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.