Rat myelin basic protein,MBP ELISA Kit

Rat myelin basic protein,MBP ELISA Kit

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中文名稱：

大鼠髓磷脂堿性蛋白(MBP)酶聯(lián)免疫試劑盒
貨號：

CSB-E08284r
規(guī)格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠髓磷脂堿性蛋白(MBP)酶聯(lián)免疫試劑盒（CSB-E08284r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MBP含量。髓鞘堿性蛋白（MBP）是一種在中樞神經(jīng)系統(tǒng)（CNS）髓鞘化過程中至關(guān)重要的蛋白質(zhì)。其研究機制涉及MBP與金屬酶的相互作用，以及作為藥物設計的靶點。通過理解MBP的結(jié)構(gòu)和功能，研究人員旨在開發(fā)新的治療方法，以診斷和治療與MBP相關(guān)的疾病，如多發(fā)性硬化癥。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于基礎醫(yī)學研究中MBP表達調(diào)控機制探索，如多發(fā)性硬化癥模型、創(chuàng)傷性腦損傷評估或神經(jīng)再生相關(guān)實驗，也可用于藥物研發(fā)過程中神經(jīng)保護效果的定量評價。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

MbpMyelin basic protein ELISA Kit; MBP ELISA Kit
縮寫：

MBP
Uniprot No.：

P02688
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-93
1:2	Average %	99
	Range %	95-103
1:4	Average %	92
	Range %	88-96
1:8	Average %	95
	Range %	91-100

回收率：

The recovery of rat MBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	95	92-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.333	2.173	2.253	2.086
10	1.402	1.377	1.390	1.223
5	0.868	0.888	0.878	0.711
2.5	0.598	0.583	0.591	0.424
1.25	0.425	0.412	0.419	0.252
0.625	0.298	0.312	0.305	0.138
0.312	0.260	0.251	0.256	0.089
0	0.168	0.166	0.167

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.
基因功能參考文獻：
1. Angiostrongylus cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-kappaB signaling pathway leading to reduced MBP production in RSC96 Schwann cells. PMID: 29742816
2. A role for the retrograde dynein/dynactin motor complex in anterograde mbp mRNA transport. PMID: 29073112
3. The aberrant phase transition of MBP molecules from their cohesive to soluble and non-adhesive state is a mechanism triggering myelin breakdown in neuromyelitis optica. PMID: 27346352
4. hindlimb unweighting treatment induced damage of L5 DRG neurons, which was correlated with decreased total MBP protein expression, increased dgen-MBP expression, and reduced GDNF protein and mRNA expression PMID: 27230884
5. Bilateral common carotid artery occlusion led to loss of myelin basic protein expression. PMID: 26670084
6. Study demonstrated that downregulation of tau severely impairs process outgrowth, oligodendrocyte differentiation, and sheet formation, and that MBP mRNA transport to the distal cellular extensions is disturbed PMID: 25847153
7. Protein levels of MBP were significantly greater in orbital frontal cortex (OFC) but not dorsal striatum (dSTR) of females as compared to males, suggesting that myelination is increased in OFC but similar in dSTR of females as compared to males PMID: 26002313
8. MBP cell membrane localization is altered in the presence of galactolipids. PMID: 25003183
9. Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4. PMID: 25512606
10. The expression of myelin basic protein was detected in accordance with the radially extending myelinated processes of the neurons in the cortex. PMID: 25282817
11. Given the principle of the longest time window and the lowest therapeutic dose, the optimized therapeutic dose and time window should be injecting picroside II intraperitoneally with 10-20 mg/kg body weight at ischemia in cerebral ischemic injury. PMID: 24524292
12. The e-MBP isoform is exclusively present in OLN-93 cells. PMID: 24321769
13. MBP epitopes normally sheltered from immunosurveillance may induce MBP-specific T cell clones and a self-sustaining immune reaction, which may together contribute to transition of acute pain into chronic neuropathic pain state. PMID: 22676642
14. Methylmercury-induced perturbation in Mbp gene expression occurred as a consequence of decreased oligodendrocyte cell population in absence of a significant impact on its post-transcriptional regulation. PMID: 22835759
15. Morphological basis of spinal cord recovery from debilitating experimental neurodenerative disease is evidenced by robust myelin basic protein (MBP) expression and extensive remyelination. PMID: 21996274
16. The impact of Hoxa2 over-expression on the proliferation and differentiation of CG4-SHoxa2 cells was investigated. PMID: 21479584
17. MBP synthesis is regulated by hnRNP-K and integrin activation. PMID: 21357748
18. Myelin basic protein expression decreases following demyelination of optic nerve and chiasm and then increases to control levels 14 days post-lesion. PMID: 20637745
19. Sp1 phosphorylation can be regulated by PKC-ERK pathways. This phosphorylation is important for MBP transcription and oligodendrocyte differentiation PMID: 20882567
20. Increasing the negative surface charge of the cell membrane reduces the amount of Fyn-SH3 domain binding to membrane-associated MBP. PMID: 19178193
21. Phosphatidylinositol-3 kinase p85 enhances expression from the myelin basic protein promoter in oligodendrocytes independent of PI3K activity and dependent on the adapter functions of the p85 subunit's SH2 domains PMID: 12390518
22. isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform. The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. PMID: 14580679
23. Two regulatory elements in the promoter interact with Nkx2.2 in vitro PMID: 15695521
24. Susceptibility or resistance to EAE is not regulated by the amount of the neuroantigens expressed in the thymus. Furthermore, the lesion distribution, but not the clinical course, of EAE is related to the neuroantigen expression in the CNS. PMID: 16773652
25. Mbp is a binding partner and calmodulin adaptor for the BKCa channel. PMID: 17610306
26. 21.5 kDa molecule expressed during [dental] hard tissue formation PMID: 18821983
27. study demonstrates co-localisation of MBP & myristoylated alanine-rich C kinase substrate (MARKS) in oligodendrocytes, & co-distribution of MBP, MARCKS & GAP-43 in lipid raft fractions recovered from myelin & brain homogenates PMID: 19026719
28. Myelin basic protein-reactive T cells persist in an inactive state in the bone marrow of Lewis rats that have recovered from autoimmune encephalomyelitis. PMID: 19211156
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亞細胞定位：

Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
蛋白家族：

Myelin basic protein family
組織特異性：

Found in both the central and the peripheral nervous system.
數(shù)據(jù)庫鏈接：

KEGG: rno:24547

STRING: 10116.ENSRNOP00000022303

UniGene: Rn.203146

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.