Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

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中文名稱：

大鼠基質金屬蛋白酶1(MMP-1/Collagenase I)酶聯免疫試劑盒
貨號：

CSB-E07416r
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠基質金屬蛋白酶1(MMP-1/Collagenase I)酶聯免疫試劑盒（CSB-E07416r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP1含量。MMP1，即基質金屬蛋白酶1，是一種鋅依賴性內肽酶，參與細胞外基質（ECM）的降解，與細胞遷移、炎癥和腫瘤侵襲等生理和病理過程密切相關。MMP1通過降解ECM中的膠原和蛋白聚糖，改變細胞微環境，促進腫瘤的侵襲和轉移。研究MMP1的機制有助于開發針對腫瘤侵襲和轉移的治療策略。試劑盒檢測范圍為0.235 ng/ml- 15 ng/ml，靈敏度為0.058 ng/ml。適用于炎癥模型、組織工程研究、纖維化疾病機制探索等科研場景，能夠為心血管疾病、關節炎等動物模型的分子機制研究提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit
縮寫：

MMP1
Uniprot No.：

P81563
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.235 ng/ml - 15 ng/ml
靈敏度：

0.058 ng/ml
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase?I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	92
	Range %	87-98
1:4	Average %	95
	Range %	90-102
1:8	Average %	100
	Range %	96-109

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.257

2.231

2.244

2.147

7.5

1.876

1.832

1.854

1.757

3.75

1.381

1.373

1.377

1.280

1.88

0.835

0.797

0.816

0.719

0.94

0.397

0.376

0.387

0.290

0.47

0.273

0.257

0.265

0.168

0.235

0.197

0.207

0.202

0.105

0.099

0.094

0.097

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Photoaging induced by long-term exposure to UV irradiation and amelioration by Skipjack tuna skin hydrolysates: Targeting inhibition of MAPK and NF-κB signaling hyperactivation D Xu,Journal of Functional Foods,2022
Dietary Supplementation with Palmitoyl-Glucosamine Co-Micronized with Curcumin Relieves Osteoarthritis Pain and Benefits Joint Mobility E Gugliandolo,Animals,2020
Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats G Shamlan,Arabian Journal for Science and Engineering,2020
Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain Cordaro M, et al,Arthritis Research & Therapy,2019
A novel composite formulation of palmitoylethanolamide and quercetin decreases inflammation and relieves pain in inflammatory and osteoarthritic pain models Britti D.et al,BMC Vet Res.,2017
Adelmidrol, in combination with hyaluronic acid, displays increased anti-inflammatory and analgesic effects against monosodium iodoacetate-induced osteoarthritis in rats Rosanna Di Paola.et al,Arthritis Research & Therapy,2016
Matrix metalloproteinases are possible targets inmonocrotaline-induced pulmonary hypertension: investigationof anti-remodeling effects of alagebrium and everolimus Atl.et al,Anatolian journal of cardiology,2016

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最新研究進展：

MMP1，全名為基質金屬蛋白酶-1，是一種廣泛存在于人體組織中的酶類分子。MMP1主要參與膠原蛋白的降解和清除過程，在組織再生、炎癥、腫瘤轉移等多種生理和病理過程中發揮重要作用。最近的研究表明，MMP1在多種癌癥的發生和發展中發揮關鍵作用，成為了一個重要的治療靶點。此外，MMP1還與關節炎、動脈粥樣硬化等疾病的發生和發展密切相關，成為了重要的生物標志物和疾病預測因子。針對這些酶的抑制劑已成為研究的熱點，可以用于疾病的預防和治療。
功能：

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. May play a role in the deterioration of the heart wall extracellular matrix proteins during the onset of dilated cardiomyopathy.
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
數據庫鏈接：

UniGene: Rn.79007

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.