Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit
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中文名稱：大鼠角化細胞內(nèi)分泌因子(KAF)/雙調(diào)蛋白(AR)酶聯(lián)免疫試劑盒
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貨號：CSB-E07299r
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規(guī)格：96T/48T
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價格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠角化細胞內(nèi)分泌因子(KAF)/雙調(diào)蛋白(AR)酶聯(lián)免疫試劑盒（CSB-E07299r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AREG含量。AREG（Amphiregulin）是一種表皮生長因子家族成員，主要在腫瘤組織中高表達，與腫瘤的增殖、侵襲和轉(zhuǎn)移密切相關。研究顯示，AREG基因的高表達與患者年齡及腫瘤細胞分化程度密切相關，其高表達可能與啟動子區(qū)去甲基化有關。此外，AREG在多種惡性腫瘤中異常活躍，如結直腸癌、非小細胞肺癌等，其過表達會加速腫瘤生長和轉(zhuǎn)移。試劑盒檢測范圍為1.25 pg/mL-80 pg/mL，該產(chǎn)品為科研領域提供可靠工具，可用于研究皮膚疾病模型、上皮損傷修復機制、慢性炎癥反應或腫瘤微環(huán)境中KAF/AR的動態(tài)變化，特別適用于動物實驗中的分子機制探索及生物標志物分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Areg; Sdgf; Amphiregulin; AR; Schwannoma-derived growth factor; SDGF
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：1.25 pg/mL-80 pg/mL
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靈敏度：0.31 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cancer
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat KAF/AR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 87-98 1:2 Average % 95 Range % 91-99 1:4 Average % 86 Range % 80-92 1:8 Average % 96 Range % 92-102 -
回收率：
The recovery of rat KAF/AR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 104 98-108 EDTA plasma (n=4) 97 92-106 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 80 2.538 2.616 2.577 2.413 40 1.939 2.059 1.999 1.835 20 1.364 1.386 1.375 1.211 10 0.882 0.870 0.876 0.712 5 0.549 0.541 0.545 0.381 2.5 0.341 0.356 0.349 0.185 1.25 0.253 0.258 0.256 0.092 0 0.168 0.160 0.164 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價
相關產(chǎn)品
靶點詳情
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功能：Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
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基因功能參考文獻：
- Report increased AREG transcript and protein levels in the lungs of rodents with DA-induced bronchiolitis obliterans. PMID: 24816162
- estrogen and progeterone increased cell proliferation in normal gland and mammary cancers through the increased production of amphiregulin PMID: 21258428
- may play a role in the ovulatory process of cycling rats as well as in the induction of ovulation in immature rats PMID: 15291759
- the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues PMID: 15509566
- findings identify Amphiregulin as a factor for resistance of glioma cells to delta9-Tetrahydrocannabinol induced apoptosis PMID: 19229996
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亞細胞定位：Membrane; Single-pass membrane protein.
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蛋白家族：Amphiregulin family
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數(shù)據(jù)庫鏈接：
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