Rat heat shock protein 27,HSP-27 ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠熱休克蛋白27(HSP-27)酶聯(lián)免疫試劑盒（CSB-E09240r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HSPB1含量。HSPB1為熱休克蛋白B1，它在細胞應(yīng)對應(yīng)激，如高溫、氧化應(yīng)激等過程中發(fā)揮重要作用。研究其機制有助于了解細胞應(yīng)激反應(yīng)、凋亡調(diào)控等生物學(xué)過程，也為相關(guān)疾病如神經(jīng)退行性疾病、癌癥等的治療提供潛在靶點。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL，適用于科研領(lǐng)域中對大鼠模型在熱應(yīng)激、神經(jīng)退行性疾病、代謝性疾病或組織損傷修復(fù)等實驗條件下的 HSP - 27 動態(tài)變化研究，為探索其分子機制及調(diào)控網(wǎng)絡(luò)提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Hspb1 ELISA Kit; Hsp27 ELISA Kit; Heat shock protein beta-1 ELISA Kit; HspB1 ELISA Kit; Heat shock 27 kDa protein ELISA Kit; HSP 27 ELISA Kit
縮寫：

HSPB1
Uniprot No.：

P42930
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-102
1:2	Average %	105
	Range %	100-113
1:4	Average %	91
	Range %	85-96
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of rat HSP-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	85	80-90

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle.
蛋白家族：

Small heat shock protein (HSP20) family
組織特異性：

Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris a
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000032902

UniGene: Rn.3841