Rat guanylate cyclase activator 2B (GUCA2B) ELISA kit

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

產(chǎn)品詳情

產(chǎn)品描述：

大鼠鳥(niǎo)苷酸環(huán)化酶激活劑2B(GUCA2B)酶聯(lián)免疫試劑盒（CSB-EL010048RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的GUCA2B含量。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，適用于基礎(chǔ)研究中GUCA2B在腸道生理機(jī)制、炎癥性腸病模型或藥物干預(yù)實(shí)驗(yàn)中的動(dòng)態(tài)變化分析，也可用于體外細(xì)胞模型中蛋白分泌水平的定量評(píng)估，為消化道疾病相關(guān)科研提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

Guca2b ELISA Kit; Guanylate cyclase activator 2B [Cleaved into: Uroguanylin ELISA Kit; UGN)] ELISA Kit
縮寫(xiě)：

GUCA2B
Uniprot No.：

P70668
種屬：

Rattus norvegicus (Rat)
樣本類(lèi)型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GUCA2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-102
1:2	Average %	92
	Range %	85-96
1:4	Average %	90
	Range %	85-94
1:8	Average %	91
	Range %	84-95

回收率：

The recovery of rat GUCA2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	81-92
EDTA plasma (n=4)	104	92-107

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.
基因功能參考文獻(xiàn)：
1. our data suggest that the inhibitory effect of UGN on NHE3 transport activity in proximal tubule is mediated by activation of both cGMP/PKG and cAMP/PKA signaling pathways PMID: 22952280
2. Uroguanylin is delivered to the kidney as an unprocessed propeptide. PMID: 18499760
3. To respond to proUGn released from the gut, any target organ must use a local mechanism for production of active peptide. PMID: 18499761
亞細(xì)胞定位：

Secreted.
蛋白家族：

Guanylin family
組織特異性：

Expressed not only in the gastrointestinal tract but also in the lung, pancreas and kidney.
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:64055

STRING: 10116.ENSRNOP00000012062

UniGene: Rn.97593