Rat growth hormone releasing hormone,GHRH ELISA Kit
-
中文名稱：大鼠促生長(zhǎng)激素釋放激素(GHRH)酶聯(lián)免疫試劑盒
-
貨號(hào)：CSB-E06878r
-
規(guī)格：96T/48T
-
價(jià)格：￥3900/￥2500
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：大鼠促生長(zhǎng)激素釋放激素(GHRH)酶聯(lián)免疫試劑盒（CSB-E06878r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GHRH含量。GHRH（生長(zhǎng)激素釋放激素）是一種由下丘腦分泌的肽類激素，主要作用是調(diào)節(jié)生長(zhǎng)激素的釋放和神經(jīng)功能。它通過激活G蛋白偶聯(lián)受體，激活cAMP信號(hào)通路，促進(jìn)細(xì)胞增殖、生長(zhǎng)激素合成與分泌，并參與免疫系統(tǒng)調(diào)節(jié)、細(xì)胞分化、細(xì)胞增殖等生物學(xué)過程。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，本產(chǎn)品廣泛應(yīng)用于神經(jīng)內(nèi)分泌研究、生長(zhǎng)激素相關(guān)疾病模型構(gòu)建、藥物干預(yù)效果評(píng)價(jià)等科研領(lǐng)域，為探索GHRH在生長(zhǎng)發(fā)育、代謝疾病或應(yīng)激反應(yīng)中的調(diào)控機(jī)制提供可靠工具。試劑盒包含完整實(shí)驗(yàn)組分，支持常規(guī)實(shí)驗(yàn)室操作流程，適用于大鼠模型的基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名：Ghrh ELISA Kit; Somatoliberin ELISA Kit; Growth hormone-releasing factor ELISA Kit; GRF ELISA Kit; Growth hormone-releasing hormone ELISA Kit; GHRH ELISA Kit
-
縮寫：GHRH
-
Uniprot No.：
-
種屬：Rattus norvegicus (Rat)
-
樣本類型：serum, plasma, tissue homogenates
-
檢測(cè)范圍：3.12 pg/mL-200 pg/mL
-
靈敏度：0.78 pg/mL
-
反應(yīng)時(shí)間：1-5h
-
樣本體積：50-100ul
-
檢測(cè)波長(zhǎng)：450 nm
-
研究領(lǐng)域：Signal Transduction
-
測(cè)定原理：quantitative
-
測(cè)定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-103 1:2 Average % 92 Range % 85-99 1:4 Average % 90 Range % 88-94 1:8 Average % 101 Range % 96-105 -
回收率：
The recovery of rat GHRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 87-95 EDTA plasma (n=4) 106 103-111 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 200 2.088 2.252 2.170 2.080 100 1.585 1.676 1.631 1.541 50 1.163 1.104 1.134 1.044 25 0.831 0.765 0.798 0.708 12.5 0.550 0.524 0.537 0.447 6.25 0.344 0.345 0.345 0.255 3.12 0.182 0.183 0.183 0.093 0 0.090 0.089 0.090 -
數(shù)據(jù)處理：
-
貨期：3-5 working days
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能：GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
-
基因功能參考文獻(xiàn)：
- GHRH agonistic analogs, JI-34 and MR-356 alleviated radiation-induced damage of cardiac myocytes. PMID: 27480202
- findings support the hypothesis that antagonizing the signaling of GHRH in T1D may improve GLP-1 function in the small intestine, which, in turn, diminishes TRL and reduces renal and vascular complications PMID: 26831066
- JI-38 had shown promising results in multiple fields including cardiology, diabetes and wound healing, our new, more potent, GHRH agonists should manifest additional efficacy for possible medical applications. PMID: 24373935
- GHRH protects the heart from ischemia/reperfusion injury through receptor-mediated mechanisms, leading to activation of RISK and SAFE pathways, which converge on mitochondria and possibly on AMP-activated protein kinase. PMID: 23417421
- We present evidence that growth retardation in upper airway obstruction is associated with a reduction in hypothalamic GHRH content. PMID: 21406516
- Growth hormone expression rises in pituitary gland during normal pregnancy, while its receptor mRNA level in hypothalamus decreases. PMID: 20814072
- GABAergic neurons in the median preoptic nucleus and ventrolateral preoptic nucleus are potential targets of the sleep-regulatory actions of GHRH. PMID: 19889861
- binding of [125I-Tyr(10)]hGHRH NH(2) to medullary homogenates was specific, time-dependent, reversible and saturable (K(d): 28 nM; B(max): 30 fmol/mg prot.). PMID: 11814616
- direct interactions between the growth hormone releasing hormone and growth hormone secretagogue receptors may explain the observed effects of of ghrelin and growth hormone secretagogues on signal transduction PMID: 12446584
- Review. Growth hormone-releasing hormone (GHRH) is a hypothalamic hormone that is essential for normal expansion of the somatotrope lineage during pituitary development. PMID: 12511850
- Changes in ghrelin and ghrelin receptor expression according to feeding status. PMID: 12876464
- Growth hormone releasing hormone (GHRH) is involved in the transcriptional regulation of the GH gene. PMID: 14664705
- results suggest that growth hormone secretagogue receptor is involved in the up-regulation of growth hormone releasing hormone and neuropeptide Y expression PMID: 16728494
- RFRP-3 infusion elicited a significant increase in growth hormone-releasing hormone mRNA and plasma growth hormone levels compared to control rats. PMID: 18628614
- Growth hormone-releasing hormone promotes survival of cardiac myocytes in vitro and protects against ischaemia-reperfusion injury in rat heart. PMID: 19293247
- Postsynaptic localization of sst(1) receptors and synaptic connectivity may be an important mechanism by which sst(1) mediates SRIF's cyclical effects on ARC GHRH neurons to generate the ultradian rhythm of GH secretion. PMID: 19553459
顯示更多
收起更多
-
亞細(xì)胞定位：Secreted.
-
蛋白家族：Glucagon family
-
數(shù)據(jù)庫(kù)鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
