Rat glutamic acid decarboxylase(GAD) autoantibody(IgG) ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠谷氨酸脫羧酶自身抗體IgG(GAD-Ab-IgG)酶聯(lián)免疫試劑盒（CSB-E08654r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的GAD auto-Ab (IgG)含量。GAD auto - Ab (IgG)即谷氨酸脫羧酶自身抗體（IgG 型），在自身免疫性疾病背景中有重要意義，如在 1 型糖尿病等疾病中常出現(xiàn)。它會攻擊表達(dá) GAD 的細(xì)胞，破壞細(xì)胞功能，影響神經(jīng)遞質(zhì)合成等過程，進(jìn)而影響機(jī)體正常生理活動。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于探索1型糖尿病、神經(jīng)系統(tǒng)疾病等動物模型中GAD-Ab-IgG的動態(tài)變化，為研究自身抗體介導(dǎo)的病理過程提供數(shù)據(jù)支持。本產(chǎn)品嚴(yán)格限定于科學(xué)研究用途，適用于基礎(chǔ)醫(yī)學(xué)、免疫學(xué)及疾病機(jī)制研究等領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

GAD auto-Ab (IgG)
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GAD autoantibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	86
	Range %	81-97
1:10	Average %	89
	Range %	85-100
1:20	Average %	95
	Range %	90-100
1:40	Average %	93
	Range %	86-99

回收率：

The recovery of rat GAD autoantibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-98
EDTA plasma (n=4)	93	87-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.