Rat glucagon-like peptide-1,GLP-1 ELISA Kit

Rat glucagon-like peptide-1,GLP-1 ELISA Kit

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中文名稱：

大鼠胰高血糖素樣肽1(Glp-1)酶聯免疫試劑盒
貨號：

CSB-E08117r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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引用文獻
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產品描述：

大鼠胰高血糖素樣肽1(Glp-1)酶聯免疫試劑盒（CSB-E08117r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的Glp1含量。Glp1即胰高血糖素樣肽-1，是腸道L細胞分泌的一種肽類激素。其背景在于可延緩胃排空、抑制食欲等。研究機制上，它能與相應受體結合，刺激胰島素分泌，降低血糖，同時還參與能量代謝調控，目前是糖尿病、肥胖癥等疾病藥物研發熱門靶點。試劑盒檢測范圍為0.625 pg/mL-40 pg/mL，該產品適用于糖尿病機制研究、代謝性疾病模型評估、腸道內分泌功能分析及藥物開發中Glp - 1相關調控效應的體外驗證，為探索胃腸激素信號通路提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

Glp1
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.625 pg/mL-40 pg/mL
靈敏度：

0.156 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	84-95
1:200	Average %	95
	Range %	91-100
1:400	Average %	84
	Range %	81-89
1:800	Average %	92
	Range %	86-98

回收率：

The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	103	95-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
40	2.607	2.744	2.676	2.531
20	2.303	2.451	2.377	2.232
10	1.817	1.922	1.870	1.725
5	1.133	1.191	1.162	1.017
2.5	0.621	0.632	0.627	0.482
1.25	0.344	0.355	0.350	0.205
0.625	0.234	0.248	0.241	0.096
0	0.140	0.150	0.145

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.