Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

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中文名稱：

大鼠脂肪酸結(jié)合蛋白4，脂肪細(xì)胞型(FABP4)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E13027r
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠脂肪酸結(jié)合蛋白4，脂肪細(xì)胞型(FABP4)酶聯(lián)免疫試劑盒（CSB-E13027r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的FABP4含量。FABP4 是一種脂肪酸結(jié)合蛋白。它在脂質(zhì)代謝和炎癥反應(yīng)等生理過程中發(fā)揮重要作用。研究機(jī)制上，F(xiàn)ABP4 參與脂肪酸的攝取、轉(zhuǎn)運(yùn)和代謝調(diào)節(jié)，其異常表達(dá)與糖尿病、肥胖癥、心血管疾病等病理狀態(tài)密切相關(guān)，是潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，可滿足細(xì)胞能量代謝研究、脂肪組織功能評(píng)估以及代謝性疾病機(jī)制探索等科研需求，例如在糖尿病模型大鼠中動(dòng)態(tài)監(jiān)測(cè)脂肪因子表達(dá)水平，或體外藥物干預(yù)實(shí)驗(yàn)中評(píng)估化合物對(duì)脂肪細(xì)胞代謝的影響。支持科研人員在體外實(shí)驗(yàn)?zāi)Ｐ椭邢到y(tǒng)研究FABP4的生物學(xué)功能及其在代謝調(diào)節(jié)網(wǎng)絡(luò)中的作用機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Fabp4Fatty acid-binding protein ELISA Kit; adipocyte ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; ALBP ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; A-FABP ELISA Kit; AFABP ELISA Kit; Fatty acid-binding protein 4 ELISA Kit
縮寫：

FABP4
Uniprot No.：

P70623
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-100
1:2	Average %	85
	Range %	80-90
1:4	Average %	89
	Range %	84-99
1:8	Average %	92
	Range %	86-101

回收率：

The recovery of rat FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	90	82-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	1.974	1.913	1.944	1.817
50	1.721	1.681	1.701	1.574
25	1.416	1.311	1.364	1.237
12.5	0.958	0.897	0.928	0.801
6.25	0.669	0.629	0.649	0.522
3.12	0.395	0.364	0.380	0.253
1.56	0.217	0.207	0.212	0.085
0	0.131	0.123	0.127

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

In utero exposure to the endocrine disruptor di-(2-ethylhexyl) phthalate induces long-term changes in gene expression in the adult male adrenal gland Martinez-Arguelles D et al,Endocrinology,2014

功能：

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
亞細(xì)胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Calycin superfamily, Fatty-acid binding protein (FABP) family
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000014701

UniGene: Rn.4258

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.