Rat deoxypyridinoline (DPD) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠脫氧膠原吡啶交聯(lián)(DPD)酶聯(lián)免疫試劑盒（CSB-E08400r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的DPD含量。DPD即二氫嘧啶脫氫酶。它是氟尿嘧啶等嘧啶類(lèi)藥物代謝關(guān)鍵酶。其活性水平影響藥物療效與毒性。研究機(jī)制主要聚焦于其基因多態(tài)性對(duì)活性的影響，以及通過(guò)調(diào)控DPD活性來(lái)優(yōu)化嘧啶類(lèi)藥物治療方案、降低不良反應(yīng)。試劑盒檢測(cè)范圍為1.56 pmol/L-100 pmol/L，適用于骨代謝動(dòng)物模型研究、抗骨質(zhì)疏松藥物療效評(píng)價(jià)、骨關(guān)節(jié)炎病理機(jī)制探索等科研領(lǐng)域，支持多種生物樣本類(lèi)型檢測(cè)，重復(fù)性良好且無(wú)需復(fù)雜前處理，為骨吸收相關(guān)基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

DPD
種屬：

Rattus norvegicus (Rat)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

1.56 pmol/L-100 pmol/L
靈敏度：

0.39 pmol/L
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	85-100
1:200	Average %	92
	Range %	87-98
1:400	Average %	89
	Range %	87-93
1:800	Average %	95
	Range %	89-99

回收率：

The recovery of rat DPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	98	92-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.