Rat collagen type Ⅱ(Col Ⅱ) antibody ELISA kit

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中文名稱：

大鼠Ⅱ型膠原(Col Ⅱ)抗體酶聯(lián)免疫試劑盒
貨號：

CSB-EQ027975RA
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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大鼠Ⅱ型膠原(Col Ⅱ)抗體酶聯(lián)免疫試劑盒（CSB-EQ027975RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Col Ⅱ Ab含量。試劑盒檢測范圍為0.312 U/mL-20 U/mL，適用于科研領(lǐng)域?qū)顷P(guān)節(jié)疾病模型的機(jī)制研究；適用于評估關(guān)節(jié)炎動(dòng)物模型中Col Ⅱ抗體的表達(dá)水平，探究軟骨退行性病變的分子機(jī)制，或用于篩選調(diào)節(jié)軟骨代謝的候選化合物；為軟骨生物學(xué)、病理學(xué)及藥物開發(fā)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

collagen type 2(Col 2) antibody,_x000D_ collagen type II(Col II) antibody
縮寫：

Col Ⅱ Ab
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma
檢測范圍：

0.312 U/mL-20 U/mL
靈敏度：

0.078 U/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-99
1:2	Average %	95
	Range %	91-100
1:4	Average %	93
	Range %	85-100
1:8	Average %	93
	Range %	82-98

回收率：

The recovery of rat Col Ⅱ antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-110
EDTA plasma (n=4)	98	89-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average	Corrected
20	2.607	2.725	2.666	2.498
10	2.036	2.081	2.059	1.891
5	1.328	1.369	1.349	1.181
2.5	0.811	0.825	0.818	0.650
1.25	0.436	0.421	0.429	0.261
0.625	0.349	0.360	0.355	0.187
0.312	0.243	0.232	0.238	0.070
0	0.169	0.167	0.168

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Evaluating the Similarity of Different Collagen-Induced Arthritis Models to the Pre-Clinical Phase of RA in Female Rats X Lin,Inflammation,2023
Guizhi-Shaoyao-Zhimu decoction attenuates rheumatoid arthritis by inhibiting mast cell degranulation L Li,Tradit Med Res,2023

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■ 常見問題解答

I have to use the CSB-EQ027975RA Rat collagen Type II (Col II) antibody kit and I have some questions:
I have to use Culture Medium not Serum or Plasma is this possible?
In other KIt we use before the Standard (CSB-EO8092r) has a concentration of 1000 pg/mL
now this new kit we are going to use has a concentration of 20 U/mL so whic is the relation between Unit and pg?. How can we calculate Collagen II concentration
Thanks in advance for your help

Thanks for your inquiry! CSB-E08092r is for quantitative determination of collagen type Ⅱ(Col Ⅱ) .
CSB-EQ027975RA is for quantitative determination of collagen type Ⅱ(Col Ⅱ) antibody.
U/mL is a quantitative unit and the standard in this kit is represented by U/mL. Sorry there is no converting relationship with pg/mL.
This kit is usually used to test serum and plasma samples. Sorry we didn't test culture medium before and we can not esure 100% if it can be detected out. We suggest you do a pretest first if you want to test it.

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Rat collagen type Ⅱ(Col Ⅱ) antibody ELISA kit

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