Rat asymmetrical dimethylarginine(ADMA) ELISA Kit

產(chǎn)品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠不對稱二甲基精氨酸(ADMA)酶聯(lián)免疫試劑盒（CSB-E13039r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADMA含量。ADMA（非對稱二甲基精氨酸）是一種內(nèi)源性一氧化氮合酶抑制劑，其水平升高與多種疾病如糖尿病視網(wǎng)膜病變和骨關(guān)節(jié)炎相關(guān)。研究顯示，ADMA通過影響細(xì)胞外基質(zhì)沉積、SOX9降解等途徑，促進疾病發(fā)展。靶向DDAH1（ADMA水解酶）可能成為治療相關(guān)疾病的新策略。試劑盒檢測范圍為1.5 ng/mL-300 ng/mL，本試劑盒適用于心血管疾病模型研究、代謝紊亂機制探索、腎臟功能評估等基礎(chǔ)科研實驗，也可用于藥物干預(yù)或基因調(diào)控實驗中ADMA水平變化的動態(tài)監(jiān)測，為動物模型病理機制解析及治療策略開發(fā)提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ADMA
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.5 ng/mL-300 ng/mL
靈敏度：

0.75 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ADMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	92
	Range %	86-96
1:4	Average %	95
	Range %	90-101
1:8	Average %	103
	Range %	99-106

回收率：

The recovery of rat ADMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	84	80-89

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.