Rat amyloid beta peptide 1-40,Aβ1-40 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠β淀粉樣蛋白1-40(Aβ1-40)酶聯(lián)免疫試劑盒（CSB-E08302r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Aβ1-40含量。Aβ1-40是重要的阿爾茨海默病相關(guān)靶點(diǎn)。Aβ蛋白異常聚集形成的斑塊是該病典型病理特征，Aβ1-40是Aβ的一種常見亞型。研究機(jī)制主要集中在揭示其產(chǎn)生、聚集過程及對(duì)神經(jīng)細(xì)胞毒性作用，以探索治療AD的新策略。試劑盒檢測(cè)范圍為0.625 pg/mL-40 pg/mL，該產(chǎn)品可廣泛應(yīng)用于神經(jīng)科學(xué)領(lǐng)域的基礎(chǔ)研究，例如評(píng)估疾病模型中Aβ1 - 40的病理積累水平、探究藥物干預(yù)對(duì)淀粉樣蛋白代謝的影響，或通過體液檢測(cè)分析個(gè)體化模型的生物標(biāo)志物動(dòng)態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
縮寫：

Aβ1-40
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.625 pg/mL-40 pg/mL
靈敏度：

0.156 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	87
	Range %	82-91
1:4	Average %	90
	Range %	85-94
1:8	Average %	105
	Range %	100-113

回收率：

The recovery of rat Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	93	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.