Rat Tissue factor,TF ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠組織因子(TF)酶聯(lián)免疫試劑盒（CSB-E07914r）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織培養(yǎng)上清液、組織勻漿、細(xì)胞裂解物樣本中的F3含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，試劑盒適用于心血管疾病模型、腫瘤生物學(xué)研究、炎癥相關(guān)實驗等科研場景，通過檢測不同樣本類型中TF的動態(tài)變化，可深入探究其在疾病發(fā)生發(fā)展中的作用機(jī)制，或用于藥物干預(yù)實驗中生物標(biāo)志物的效果評估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

F3 ELISA Kit; Cf3 ELISA Kit; Tissue factor ELISA Kit; TF ELISA Kit; Coagulation factor III ELISA Kit; CD antigen CD142 ELISA Kit
縮寫：

F3
Uniprot No.：

P42533
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, urine, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Blood Coagulation
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	91-106
1:2	Average %	90
	Range %	85-96
1:4	Average %	93
	Range %	89-100
1:8	Average %	99
	Range %	93-108

回收率：

The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-94
EDTA plasma (n=4)	103	95-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Tissue factor family
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000015836

UniGene: Rn.9980