Rat Tenascin-C(TN-C) ELISA Kit
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中文名稱：大鼠粘膠蛋白/肌腱蛋白C(TN-C)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E13377r
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠粘膠蛋白/肌腱蛋白C(TN-C)酶聯(lián)免疫試劑盒（CSB-E13377r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TN-C含量。TN - C是當(dāng)前靶點(diǎn)。它在細(xì)胞外基質(zhì)中發(fā)揮重要作用，與細(xì)胞黏附、遷移等過(guò)程相關(guān)。研究其機(jī)制聚焦于它和腫瘤微環(huán)境、腫瘤細(xì)胞生長(zhǎng)及轉(zhuǎn)移的關(guān)系，通過(guò)揭示TN - C作用機(jī)制或?yàn)槟[瘤等相關(guān)疾病的治療提供新靶點(diǎn)與思路。試劑盒檢測(cè)范圍為2.5 ng/mL-160 ng/mL，科研人員可將其應(yīng)用于心血管疾病模型、肝臟纖維化研究或創(chuàng)傷修復(fù)機(jī)制探索，通過(guò)檢測(cè)不同病理狀態(tài)下TN - C的動(dòng)態(tài)表達(dá)，揭示其在細(xì)胞黏附、信號(hào)轉(zhuǎn)導(dǎo)中的功能，為靶向治療策略提供基礎(chǔ)數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：TN-C
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種屬：Rattus norvegicus (Rat)
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樣本類(lèi)型：serum, plasma, tissue homogenates
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檢測(cè)范圍：2.5 ng/mL-160 ng/mL
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靈敏度：0.625 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Neuroscience
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat TN-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 84 Range % 80-88 1:4 Average % 106 Range % 101-113 1:8 Average % 93 Range % 89-98 -
回收率：
The recovery of rat TN-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-100 EDTA plasma (n=4) 88 84-92 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 160 2.417 2.502 2.460 2.301 80 2.102 2.173 2.138 1.979 40 1.700 1.615 1.658 1.499 20 1.085 1.049 1.067 0.908 10 0.671 0.684 0.678 0.519 5 0.424 0.437 0.431 0.272 2.5 0.293 0.281 0.287 0.128 0 0.162 0.156 0.159 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評(píng)價(jià)
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