Rat Retinoic acid receptor responder protein 2(RARRES2) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠維甲酸受體應(yīng)答蛋白2(RARRES2)酶聯(lián)免疫試劑盒（CSB-EL019342RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的RARRES2含量。大鼠維甲酸受體應(yīng)答蛋白2（RARRES2）是一種調(diào)節(jié)脂肪生成和脂質(zhì)代謝的脂肪因子，其在癌癥發(fā)生發(fā)展中具有腫瘤抑制作用。研究顯示，RARRES2通過調(diào)節(jié)脂質(zhì)代謝重編程，在乳腺癌腦轉(zhuǎn)移中發(fā)揮關(guān)鍵作用，并可能成為潛在的治療靶點。試劑盒檢測范圍為0.9 ng/mL-60 ng/mL，適用于實驗室環(huán)境下開展代謝調(diào)控研究、脂肪細(xì)胞分化實驗、炎癥相關(guān)信號通路探索等科研項目。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

HP10433, TIG2, chemerin
縮寫：

RARRES2
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.9 ng/mL-60 ng/mL
靈敏度：

0.225 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat RARRES2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	96
	Range %	93-99
1:10	Average %	83
	Range %	80-86
1:20	Average %	87
	Range %	83-90
1:40	Average %	100
	Range %	97-103

回收率：

The recovery of rat RARRES2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-90
EDTA plasma (n=4)	95	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.