Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

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中文名稱：

大鼠肺表面活性物質(zhì)相關(guān)蛋白B(SP-B)酶聯(lián)免疫試劑盒
貨號：

CSB-E12630r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠肺表面活性物質(zhì)相關(guān)蛋白B(SP-B)酶聯(lián)免疫試劑盒（CSB-E12630r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFTPB含量。SP-B是肺表面活性物質(zhì)的重要組成部分，參與調(diào)節(jié)肺泡表面張力，維持呼吸功能。其研究機制涉及炎癥反應(yīng)、氧化應(yīng)激，與肺發(fā)育障礙密切相關(guān)。近年來，深度學習等技術(shù)在藥物-靶標相互作用預(yù)測方面取得進展，為SP-B相關(guān)疾病研究提供新思路。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，可廣泛用于基礎(chǔ)科研中肺部疾病機制研究、藥物干預(yù)效果評估或動物模型（如肺損傷、肺纖維化及新生兒呼吸窘迫綜合征）的SP - B動態(tài)監(jiān)測，為探索SP - B在呼吸生理及病理過程中的調(diào)控作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
縮寫：

SFTPB
Uniprot No.：

P22355
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	85-100
1:2000	Average %	94
	Range %	87-98
1:4000	Average %	90
	Range %	85-96
1:8000	Average %	94
	Range %	90-98

回收率：

The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	91	85-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.470	2.589	2.530	2.377
100	2.115	2.124	2.120	1.967
50	1.713	1.726	1.720	1.567
25	1.315	1.356	1.336	1.183
12.5	0.985	0.971	0.978	0.825
6.25	0.532	0.541	0.537	0.384
3.12	0.378	0.389	0.384	0.231
0	0.152	0.154	0.153

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
基因功能參考文獻：
1. SP-B is present on the surface of exocytosed surfactant particles. Antibodies against SP-B stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-B acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
2. Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats PMID: 26196539
3. The role of the SP-B NH(2) terminus in the formation and stability of cylindrical structures, specifically the distribution of lipid stack height, width, and density with four SP-B truncation peptides, was studied. PMID: 20023175
4. VEGF increased expression of SP-b mRNA in preterm rat lung. PMID: 17267143
5. alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time PMID: 18344412
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亞細胞定位：

Secreted, extracellular space, surface film.
數(shù)據(jù)庫鏈接：

KEGG: rno:192155

STRING: 10116.ENSRNOP00000014505

UniGene: Rn.1952

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.