Rat Protein S100-A6(S100A6) ELISA kit

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中文名稱：

大鼠S100-A6蛋白(S100A6)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL020634RA
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

大鼠S100-A6蛋白(S100A6)酶聯(lián)免疫試劑盒（CSB-EL020634RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的S100A6含量。S100A6是一個(gè)重要靶點(diǎn)。它在多種生理和病理過程中有作用，如細(xì)胞的增殖、分化等。相關(guān)研究聚焦于其與疾病發(fā)生發(fā)展的關(guān)聯(lián)機(jī)制，試圖明確其在信號(hào)通路中的角色，為開發(fā)針對(duì)該靶點(diǎn)的治療方法提供理論基礎(chǔ)。試劑盒檢測范圍為2.4 ng/mL-150 ng/mL，可廣泛應(yīng)用于神經(jīng)生物學(xué)、腫瘤機(jī)制研究及炎癥反應(yīng)等領(lǐng)域的科研實(shí)驗(yàn)，例如在動(dòng)物模型中分析S100A6在不同疾病狀態(tài)下的動(dòng)態(tài)表達(dá)水平，或探究其在細(xì)胞應(yīng)激反應(yīng)中的調(diào)節(jié)作用。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

S100A6 ELISA Kit; Protein S100-A6 ELISA Kit; Calcyclin ELISA Kit; Lung 10 kDa protein ELISA Kit; S100 calcium-binding protein A6 ELISA Kit
縮寫：

S100A6
Uniprot No.：

P30801
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

2.4 ng/mL-150 ng/mL
靈敏度：

0.6 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	84
	Range %	80-88
1:4	Average %	107
	Range %	103-110
1:8	Average %	112
	Range %	109-115

回收率：

The recovery of rat S100A6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	95	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
150	2.700	2.765	2.733	2.570
75	1.985	1.935	1.960	1.797
37.5	1.341	1.320	1.331	1.168
18.75	0.870	0.868	0.869	0.706
9.4	0.526	0.541	0.534	0.371
4.7	0.395	0.382	0.389	0.226
2.4	0.247	0.235	0.241	0.078
0	0.162	0.164	0.163

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Single‐Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage X Wang,Advanced science,2023
Differential proteomic analysis of dimethylnitrosamine (DMN)-induced liver fibrosis. Liu X.et al,Proteomics.,2017

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靶點(diǎn)詳情

功能：

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
亞細(xì)胞定位：

Nucleus envelope. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

S-100 family
數(shù)據(jù)庫鏈接：

KEGG: ocu:100348755

STRING: 9986.ENSOCUP00000017711

UniGene: Ocu.1714

Rat Protein S100-A6(S100A6) ELISA kit

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