Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit

Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit

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中文名稱：

大鼠磷酸肌醇3激酶(PI3K)酶聯免疫試劑盒
貨號：

CSB-E08418r
規格：

96T/48T
價格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

大鼠磷酸肌醇3激酶(PI3K)酶聯免疫試劑盒（CSB-E08418r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PI3K含量。PI3K（磷脂酰肌醇-3激酶）是一種脂類激酶，在癌癥發生發展中起著關鍵作用。其研究背景源于其在細胞信號傳導中的重要作用，特別是在PI3K/Akt/mTOR信號通路中，該通路調控細胞生長、存活、代謝和血管生成。PI3K通過磷酸化脂質分子PI-3,4,5-P3（PIP3）來激活下游信號分子，如Akt和mTOR，從而促進腫瘤生長和抵抗治療。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產品可廣泛應用于基礎科研領域，包括但不限于腫瘤微環境研究、代謝性疾病模型構建、藥物作用機制探索等實驗場景，為PI3K相關信號通路分析及分子調控研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PI3K
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-95
1:2	Average %	103
	Range %	99-107
1:4	Average %	93
	Range %	88-94
1:8	Average %	99
	Range %	95-103

回收率：

The recovery of rat PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	96	91-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.860	2.778	2.819	2.679
500	2.231	2.222	2.227	2.087
250	1.379	1.404	1.392	1.252
125	0.748	0.761	0.755	0.615
62.5	0.464	0.448	0.456	0.316
31.2	0.307	0.302	0.305	0.165
15.6	0.218	0.211	0.215	0.075
0	0.141	0.139	0.140

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Role of VEGFA in type 2 diabetes mellitus rats subjected to partial hepatectomy C Rojano-Alfonso, M Micó-Carnero, C Maroto-Serrat,npj Gut and Liver,2024
The role of Neuregulin-1 in steatotic and non-steatotic liver transplantation from donors after cardiocirculatory death Casillas-Ramírez, C Maroto-Serrat,npj Gut and Liver,2024
Involvement of PI3K/HIF-1α/c-MYC/iNOS Pathway in the Anticancer Effect of Suaeda vermiculata in Rats HA Mohammed,Pharmaceuticals,2023
Klotho inhibits IGF1R/PI3K/AKT signalling pathway and protects the heart from oxidative stress during ischemia/reperfusion injury A Olejnik,Scientific Reports,2023
Preventive empagliflozin activity on acute acetic acid-induced ulcerative colitis in rats via modulation of SIRT-1/PI3K/AKT pathway and improving colon barrier MS Zaghloul,Environmental toxicology and pharmacology,2023
Curcumin ameliorate cortical histomorphological deficits in Streptozocin and western diet induced neurodegeneration in Wistar Rats AA Mohammed,ResearchSquare,2022
Upregulation of HMGB1 secretion in lungs of pigs infected by highly pathogenic porcine reproductive and respiratory syndrome virus R Wang,Veterinary Microbiology,2020
Pharmacological Manipulation of Trk, p75NTR, and NGF Balance Restores Memory Deficit in Global Ischemia/Reperfusion Model in Rats Choucry AM, et al,Journal of molecular neuroscience,2019
Alpha lipoic acid protects against dexamethasone-induced metabolic abnormalities via APPL1 and PGC-1 α up regulation Mohammed MA, et al,Steroids,2019
Neuroprotective effect of vildagliptin against cerebral ischemia in rats Salma A. El-Marasy.et al,Naunyn-Schmiedeberg\'s Archives of Pharmacology,2018

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.